Introducing clinical nanoarchaeaology: Isolation by co-culture of Nanopusillus massiliensis sp. nov
| Autor: | Hassani, Y., Saad, J., Terrer, E., Aboudharam, G., Giancarlo, B, Silvestri, F., Raoult, D., Drancourt, M., Grine, G. |
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| Přispěvatelé: | Anthropologie bio-culturelle, Droit, Ethique et Santé (ADES), Aix Marseille Université (AMU)-EFS ALPES MEDITERRANEE-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
Nanoarchaea Nanopusillus massiliensis QH426-470 Classification digestive system [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Microbiology QR1-502 Oral cavity stomatognathic diseases fluids and secretions Infectious Diseases Immunology and Microbiology (miscellaneous) Genetics ComputingMilieux_MISCELLANEOUS Research Paper major categories |
| Zdroj: | Current Research in Microbial Sciences Current Research in Microbial Sciences, 2022, 3, pp.100100. ⟨10.1016/j.crmicr.2021.100100⟩ Current Research in Microbial Sciences, Vol 3, Iss, Pp 100100-(2022) |
| ISSN: | 2666-5174 |
| Popis: | Highlights • The first ever detection in human microbiota of nanoarchaea. • Detection and co-isolation of nanoarchaea new species in human oral microbiota. • These data suggest the contribution of methanogens to the perinatal development of intestinal microbiota and physiology. • Extended our knowledge of human microbiota diversity. • Opening a new field of research in clinical microbiology here referred to as clinical nanoarchaeology. Background Nanoarchaeota, obligate symbiont of some environmental archaea with reduced genomes, have been described in marine thermal vent environments, yet never detected in hosts, including humans. Methods Here, using laboratory tools geared towards the detection of nanoarchaea including PCR-sequencing, WGS, microscopy and culture. Results We discovered a novel nanoarchaea, Nanopusillus massiliensis, detected in dental plate samples by specific PCR-based assays. Combining fluorescent in situ hybridization (FISH) with scanning electron microscopy disclosed close contacts between N. massiliensis and the archaea Methanobrevibacter oralis in these samples. Culturing one sample yielded co-isolation of M. oralis and N. massiliensis with a 606,935-bp genome, with 23.6% GC encoded 16 tRNA, 3 rRNA and 942 coding DNA sequences, of which 400 were assigned to clusters of orthologous groups. Conclusion The discovery of N. massiliensis, made publicly available in collection, extended our knowledge of human microbiota diversity, opening a new field of research in clinical microbiology here referred to as clinical nanoarchaeology. |
| Databáze: | OpenAIRE |
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