Impact of dose-rate on the low-dose hyper-radiosensitivity and induced radioresistance (HRS/IRR) response

Autor: Jennifer H. Martin, Clément Devic, Michel Diserbo, Juliette Thariat, Charles Thomas, Nicolas Foray, Elke Bräuer-Krisch
Přispěvatelé: Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), European Synchrotron Radiation Facility (ESRF), IRBA, Institut de Recherche Biomédicale des Armées (IRBA), Centre de Lutte contre le Cancer Antoine Lacassagne [Nice] (UNICANCER/CAL), UNICANCER-Université Côte d'Azur (UCA), Thomas, Charles, groupe Foray-équipe Ansiau/Puisieux, Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Microbeam Radiation Therapy, Unité de Radiothérapie, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre de Lutte contre le Cancer Antoine Lacassagne [Nice] (UNICANCER/CAL), Université Côte d'Azur (UCA)-UNICANCER-Université Côte d'Azur (UCA)-UNICANCER-Centre de Lutte contre le Cancer, E02, Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université Nice Sophia Antipolis (1965 - 2019) (UNS), UNICANCER-Université Côte d'Azur (UCA)-UNICANCER-Université Côte d'Azur (UCA)-Centre de Lutte contre le Cancer
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Clonogenic survival
DNA Repair
MESH: DNA Breaks
Double-Stranded
Radiation Tolerance
DSB repair
030218 nuclear medicine & medical imaging
0302 clinical medicine
DNA Breaks
Double-Stranded
MESH: Animals
Cobalt Radioisotopes
MESH: Cobalt Radioisotopes
MESH: DNA Repair
MESH: Radiation Tolerance
Radiological and Ultrasound Technology
Chemistry
Low dose
030220 oncology & carcinogenesis
Dna breaks
Hyper-radiosensitivity (HRS) response
MESH: Radiation Dosage
MESH: Cell Line
Tumor
MESH: Rats
Colon
induced radioresistance (IRR) response
tumor cells
[SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine
Radiation Dosage
[SDV.IB.MN] Life Sciences [q-bio]/Bioengineering/Nuclear medicine
Andrology
03 medical and health sciences
MESH: X-Rays
Radioresistance
Cell Line
Tumor
Animals
Humans
Radiology
Nuclear Medicine and imaging
Radiosensitivity
MESH: Colon
radiotherapy
dose-rate
MESH: Humans
business.industry
X-Rays
MESH: Gamma Rays
Rats
Cell culture
Gamma Rays
Hyper radiosensitivity
Dose rate
Nuclear medicine
business
Zdroj: International Journal of Radiation Biology
International Journal of Radiation Biology, 2013, 89 (10), pp.813-22. ⟨10.3109/09553002.2013.800248⟩
International Journal of Radiation Biology, Informa Healthcare, 2013, 89 (10), pp.813-22. ⟨10.3109/09553002.2013.800248⟩
ISSN: 0955-3002
1362-3095
DOI: 10.3109/09553002.2013.800248⟩
Popis: International audience; PURPOSE: To ask whether dose-rate influences low-dose hyper- radiosensitivity and induced radioresistance (HRS/IRR) response in rat colon progressive (PRO) and regressive (REG) cells. METHODS: Clonogenic survival was applied to tumorigenic PRO and non-tumorigenic REG cells irradiated with (60)Co γ-rays at 0.0025-500 mGy.min(-1). Both clonogenic survival and non-homologous end-joining (NHEJ) pathway involved in DNA double-strand breaks (DSB) repair assays were applied to PRO cells irradiated at 25 mGy.min(-1) with 75 kV X-rays only. RESULTS: Irrespective of dose-rates, marked HRS/IRR responses were observed in PRO but not in REG cells. For PRO cells, the doses at which HRS and IRR responses are maximal were dependent on dose-rate; conversely exposure times during which HRS and IRR responses are maximal (t(HRSmax) and t(IRRmax)) were independent of dose-rate. The t(HRSmax) and t(IRRmax) values were 23 ± 5 s and 66 ± 7 s (mean ± standard error of the mean [SEM], n = 7), in agreement with literature data. Repair data show that t(HRSmax) may correspond to exposure time during which NHEJ is deficient while t(IRRmax) may correspond to exposure time during which NHEJ is complete. CONCLUSION: HRS response may be maximal if exposure times are shorter than t(HRSmax) irrespective of dose, dose-rate and cellular model. Potential application of HRS response in radiotherapy is discussed.
Databáze: OpenAIRE
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