Linkage map construction involving a reciprocal translocation
| Autor: | A. Farre, L. Cistué, I. Lacasa Benito, Johannes Jansen, J.H. de Jong, Ignacio Romagosa |
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| Rok vydání: | 2010 |
| Předmět: |
Heterozygote
DNA Plant Genetic Linkage rflp map breakpoints integration Chromosomal translocation length Biology Laboratorium voor Erfelijkheidsleer Genome Chromosomes Plant Translocation Genetic Gene mapping Genetic linkage Genetics chromosome 3h genome Recombination Genetic isozyme Linkage (software) Original Paper Breakpoint Chromosome Mapping Hordeum General Medicine microsatellite markers rye Biometris Laboratory of Genetics Hordeum vulgare Agronomy and Crop Science Genome Plant barley hordeum-vulgare Recombination Biotechnology |
| Zdroj: | Theoretical and Applied Genetics, 122(5), 1029-1037 Digital.CSIC. Repositorio Institucional del CSIC instname TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik Theoretical and Applied Genetics 122 (2011) 5 |
| ISSN: | 1432-2242 0040-5752 |
| Popis: | 33 Pag., 5 Fig. The definitive version is available at: http://www.springerlink.com/content/0040-5752/ This paper is concerned with a novel statistical–genetic approach for the construction of linkage maps in populations obtained from reciprocal translocation heterozygotes of barley (Hordeum vulgare L.). Using standard linkage analysis, translocations usually lead to ‘pseudo-linkage’: the mixing up of markers from the chromosomes involved in the translocation into a single linkage group. Close to the translocation breakpoints recombination is severely suppressed and, as a consequence, ordering markers in those regions is not feasible. The novel strategy presented in this paper is based on (1) disentangling the “pseudo-linkage” using principal coordinate analysis, (2) separating individuals into translocated types and normal types and (3) separating markers into those close to and those more distant from the translocation breakpoints. The methods make use of a consensus map of the species involved. The final product consists of integrated linkage maps of the distal parts of the chromosomes involved in the translocation. This study has been supported by the Spanish Ministry of Science and Innovation (Project AGL2008-05541-C02 and the Agrogenomics CONSOLIDER). |
| Databáze: | OpenAIRE |
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