Estimation of Extracellular Matrix Production Using a Cultured-Chondrocyte-Based Gate Ion-Sensitive Field-Effect Transistor
| Autor: | Hiroto Satake, Toshiya Sakata |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Transistors
Electronic Biosensing Techniques Deoxyglucose 010402 general chemistry 01 natural sciences Chondrocyte Analytical Chemistry law.invention Extracellular matrix Chondrocytes law medicine Animals Cells Cultured Glycosaminoglycans biology Chemistry 010401 analytical chemistry Transistor Ion sensitive Acid phosphatase Biological activity Hydrogen-Ion Concentration Extracellular Matrix Nanostructures 0104 chemical sciences Hydroxyproline medicine.anatomical_structure biology.protein Biophysics Cattle Colorimetry Field-effect transistor ISFET |
| Zdroj: | Analytical Chemistry. 91:16017-16022 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.9b04789 |
| Popis: | Autologous chondrocytes are generally evaluated by mechanical, histological, and biochemical methods. However, the analyzed data are from discontinuous measurements at an end point, during which living chondrocytes are destroyed and stained. To solve the problem, we developed a label-free and noninvasive method of estimating extracellular matrix (ECM) production that results from chondrocyte metabolic activities using a cultured-chondrocyte-based gate ion-sensitive field-effect transistor (ISFET) in this study. Using the cultured-chondrocyte-based gate ISFET sensor, we found that the change in electrical signal (ΔVout), which indicated the change in interfacial pH at the chondrocyte/gate nanogap (ΔpHint), gradually increased over 3 weeks upon adding a biologically active substance, l-ascorbic acid phosphate magnesium salt n-hydrate (APM), which was attributable to chondrocyte metabolic activities. The increase in ΔVout (ΔpHint) caused by the chondrocyte metabolic activities was also confirmed by the obser... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|