Bisphenol B stimulates Leydig cell proliferation but inhibits maturation in late pubertal rats
| Autor: | Zengqiang Li, Ren-Shan Ge, Haoni Yan, Song Zhang, Lili Tian, Feifei Ma, Xiu Xin, Yige Yu, Cheng Zou, Yang Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Down-Regulation Toxicology 03 medical and health sciences 0404 agricultural biotechnology Phenols Internal medicine medicine Animals Testosterone Estrogens Non-Steroidal Sexual Maturation Benzhydryl Compounds Gonadal Steroid Hormones 030304 developmental biology Cell Proliferation 0303 health sciences biology Leydig cell Chemistry Cell Cycle Leydig Cells 04 agricultural and veterinary sciences General Medicine Cell cycle Luteinizing Hormone Epididymis Sertoli cell 040401 food science Proliferating cell nuclear antigen Rats medicine.anatomical_structure Endocrinology HSD3B1 biology.protein Follicle Stimulating Hormone Food Science Hormone |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 153 |
| ISSN: | 1873-6351 |
| Popis: | Bisphenol B (BPB) has been used as a substitute for bisphenol A (BPA) in plastic materials. Whether BPB disrupts the male reproductive system remains unknown. Here, we report the effect of BPB on Leydig cell maturation in late puberty. Male Sprague-Dawley (35 days old) rats were gavaged with BPB at 0, 10, 100, and 200 mg/kg/day for 21 days. BPB significantly reduced body and epididymis weight at 200 mg/kg. BPB markedly decreased serum testosterone levels at 100 and 200 mg/kg and serum luteinizing hormone and follicle-stimulating hormone levels at 200 mg/kg. BPB significantly increased Leydig cell number at 100 and 200 mg/kg, while down-regulating the expression of Leydig cell genes (Cyp11a1 and Hsd3b1) at ≥100 mg/kg and up-regulating the expression of Sertoli cell genes (Pdgfra, Fshr, Sox9) and cell cycle regulators (Pcna, Ccnb1, Cdk2, and Cdk4) at 10–200 mg/kg. BPB markedly increased the phosphorylation of AKT1, AKT2, and ERK1/2 at 200 mg/kg. BPB increased the proliferation of rat immature Leydig cells via promoting the S/M2 phase shift at 100 and 1000 nM after 24-h culture in vitro. In conclusion, BPB disrupts Leydig cell maturation in late puberty by increasing Leydig cell number while inhibiting its maturation. |
| Databáze: | OpenAIRE |
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