The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni
| Autor: | D. R. A. Wareing, Andrew J. Fox, C. J. Jackson, D. N. Hutchinson, D.M. Jones |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Genotype Epidemiology Biology Campylobacter jejuni Disease Outbreaks Ribotyping Campylobacter Infections Humans Typing Serotyping Genotyping Techniques Genotyping Genetics Molecular Epidemiology Molecular epidemiology Chromosome Mapping Discriminant Analysis biology.organism_classification Phenotype Infectious Diseases Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Research Article |
| Zdroj: | Epidemiology and Infection. 117:233-244 |
| ISSN: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268800001400 |
| Popis: | SummaryCampylobacter jejuniserogroup reference strains and collections of sporadic and outbreak- associated isolates were examined for restriction fragment length polymorphisms (RFLPs), usingC. jejunirandom chromosomal and 16S rRNA gene probes. A collection of 48 Penner (HS) and 14 Lior (HL) serogroup reference strains, plus 10 clinical isolates, generated 35 RFLP and 26 ribotype patterns. In combination the two loci generated 48 distinct genotypes. Both probes were able to differentiate between certain random isolates of the same HS/HL serogroups but greater discrimination was obtained with RFLP than with ribotyping. Genotyping distinguished accurately between related and unrelated strains when applied to several outbreaks. Genotypic analysis ofC. jejuniby restriction fragment length polymorphisms is a valuable technique for epidemiological typing. Chromosomal variation detected by the two unlinked probe loci provides some information about the genetic relationship between isolates. |
| Databáze: | OpenAIRE |
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