Analysis of tropomyosin cDNAs isolated from skeletal and smooth muscle mRNA
| Autor: | Gary J. Lindquester, Sally Berish, James E. Flach, Kathryn H. Hickman, Robert B. Devlin |
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| Rok vydání: | 1986 |
| Předmět: |
macromolecular substances
Tropomyosin Biology Quail Genetics medicine Animals Amino Acid Sequence RNA Messenger Cloning Molecular Gene chemistry.chemical_classification Messenger RNA Base Sequence cDNA library Muscles RNA Skeletal muscle Muscle Smooth DNA Molecular biology Amino acid medicine.anatomical_structure chemistry RNA splicing |
| Zdroj: | Nucleic acids research. 14(22) |
| ISSN: | 0305-1048 |
| Popis: | Several clones containing alpha tropomyosin sequences were isolated from cDNA libraries prepared from quail skeletal or smooth muscle RNA. All of these clones contain identical sequences coding for amino acids 81-257 of alpha skeletal muscle tropomyosin where they overlap, strongly indicating they are derived from the same gene. However, there are differences among these clones in sequences coding for the final 27 amino acids, as well as 3' untranslated sequences. In addition, S1 nuclease and Northern analyses indicate that coding sequences at the 5' end of the tropomyosin mRNA are also differentially expressed in smooth and skeletal muscle. These data point to the likelihood that differential splicing at both the 5' and 3' ends of a single tropomyosin gene results in the appearance of multiple tissue-specific transcripts. |
| Databáze: | OpenAIRE |
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