TOXINS, PUTATIVE CELL ADHESINS AND FIBRONECTIN BINDING PROPERTIES OF SALMONELLA DUBLIN
| Autor: | Jens Laurits Larsen, Karina Kristiansen, Suraj B. Baloda, Torkel Wadström |
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| Rok vydání: | 2009 |
| Předmět: |
Salmonella
Hemagglutination Swine Bacterial Toxins Fimbria Enterotoxin Biology medicine.disease_cause Microbiology Bacterial Adhesion Cell Line Enterotoxins medicine Animals Humans General Immunology and Microbiology Cytotoxins Toxin Chinese hamster ovary cell General Medicine Fibronectins Bacterial adhesin Fibronectin binding Fimbriae Bacterial Cattle Female |
| Zdroj: | Acta Pathologica Microbiologica Scandinavica Series B: Microbiology. :57-63 |
| ISSN: | 0108-0180 |
| DOI: | 10.1111/j.1699-0463.1987.tb03087.x |
| Popis: | Fifty Salmonella dublin strains isolated from cattle and human diarrhoeal cases were assayed for toxin production, haemagglutination, cell-surface hydrophobicity and fibronectin-binding properties. Most strains (65% of tested) produced cytotonic toxins and cytotoxic factors when tested on Chinese hamster ovary (CHO) cells and rabbit skin test. However, only three strains produced a skin-permeability factor as determined in pig skin intra-dermal tests. None of the strains were positive in pig intestinal loop tests. Six of the 32 strains tested for 125I-fibronectin and its 125I-29 kDa N-terminal domain binding showed 10-17% and 6-10% binding, respectively. Most of the strains expressed mannose-sensitive haemagglutination (MSHA) (76%) and high cell-surface hydrophobicity (74%) when grown at 37 degrees C. At 20 degrees C the expression of MSHA and especially the expression of high cell-surface hydrophobicity were reduced. Twelve strains grown at 37 degrees C did not haemagglutinate erythrocytes from five animal species used in this study, while six of these strains expressed high cell-surface hydrophobicity. Salmonella dublin strains isolated in Denmark appeared to express a higher frequency of fimbriae type 1 (MSHA) and a lower frequency of high cell-surface hydrophobicity than the strains from external sources. |
| Databáze: | OpenAIRE |
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