Expression pattern of vascular endothelial growth factor 2 during sea urchin development
| Autor: | Nelly A. Odintsova, Konstantin V. Yakovlev, Milana A. Kulakova, Yulia O. Kipryushina |
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| Rok vydání: | 2013 |
| Předmět: |
animal structures
Mesenchyme Molecular Sequence Data Gene Expression In situ hybridization Complementary DNA biology.animal Genetics medicine Animals Amino Acid Sequence Pluteus Molecular Biology Sea urchin Phylogeny Strongylocentrotus biology Vascular Endothelial Growth Factors urogenital system Gene Expression Regulation Developmental Embryo Anatomy biology.organism_classification Strongylocentrotus purpuratus Cell biology Gastrulation medicine.anatomical_structure Organ Specificity embryonic structures Developmental Biology |
| Zdroj: | Gene Expression Patterns. 13:402-406 |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.gep.2013.07.003 |
| Popis: | The VEGF family in the sea urchin is comprised of three members designated Vegf1 through Vegf3. In this study, we found a high level of similarity between the PDGF/VEGF domain of the predicted gene Sp-Vegf2 in the sea urchin Strongylocentrotus purpuratus and the same domain of a gene that we found in a closely related sea urchin, Strongylocentrotus intermedius. The sequence of the Si-Vegf2 cDNA was determined, and the expression of the Si-Vegf2 mRNA throughout early sea urchin development was studied by RT-PCR and in situ hybridization. Also we analyzed phylogenetic relationships of Si-Vegf2 and other members of the PDGF and VEGF families. We have found that the Si-Vegf2 present during the time span from the egg to the 4-arm pluteus stage. This mRNA is uniformly distributed in eggs, cleaving embryos and early blastulae. At the gastrula stage, the Si-Vegf2 transcripts are localized in the ventrolateral clusters of primary mesenchyme cells, and later, at the prism stage, they are detected in the forming apex. At the early pluteus stage, Si-Vegf2 mRNAs are found in two groups of mesenchyme cells in the scheitel region on the apical pole. We have determined that Si-Vegf2 is a mesenchyme-expressed factor but its developmental function is unknown. |
| Databáze: | OpenAIRE |
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