Glucose-6-phosphate dehydrogenase supports the functioning of the synapses in rat cerebellar cortex
| Autor: | Loretta Guidi, Patrizia Ambrogini, Stefano Papa, Paolino Ninfali, Samuela Capellacci, Enrica Biagiotti, Paolo Del Grande |
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| Rok vydání: | 2001 |
| Předmět: |
Male
congenital hereditary and neonatal diseases and abnormalities Cerebellum Neurite Glucosephosphate Dehydrogenase Biology Synaptic Transmission Rats Sprague-Dawley Synapse Cerebellar Cortex Purkinje Cells chemistry.chemical_compound Neurochemical Basket cell hemic and lymphatic diseases parasitic diseases medicine Animals Glucose-6-phosphate dehydrogenase Molecular Biology Neurons General Neuroscience nutritional and metabolic diseases Dehydroepiandrosterone Granule cell Immunohistochemistry Rats Cell biology Microscopy Electron Glucose medicine.anatomical_structure Biochemistry chemistry Spectrophotometry Cerebellar cortex Synapses Neurology (clinical) Energy Metabolism Developmental Biology |
| Popis: | This study investigates heterogeneous glucose-6-phosphate dehydrogenase (G6PD) expression in the rat cerebellar cortex. G6PD activity and its electrophoretic pattern, evaluated on the cerebellar homogenate, were found to be similar to those of other brain areas. However, histochemical and immunohistochemical analyses revealed that the highest expression of G6PD activity and protein was in Purkinje's cells, followed by the molecular and granular layers. Electron microscopy analysis showed that, in Purkinje's cells, the G6PD reaction products were concentrated in the neurites while in the basket cells in the cell body. The granules showed a weaker activity everywhere. The quantitative distribution of G6PD is discussed in the light of the neurochemical function of these cells. |
| Databáze: | OpenAIRE |
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