Identification of Apolipoprotein A-I as a Retinoic Acid-binding Protein in the Eye
| Autor: | Marcela Hermann, Hanke Van-Der-Wel, Jennifer N. Byrum, Angelica R. Harper, Jody A. Summers, Christopher M. West, Christa L. Feasley |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
genetic structures Apolipoprotein B Receptors Retinoic Acid Retinoic acid Tretinoin Retinoic acid receptor beta Biology Biochemistry Avian Proteins 03 medical and health sciences chemistry.chemical_compound Animals Retinoic acid binding Eye Proteins Molecular Biology Apolipoprotein A-I 030102 biochemistry & molecular biology Choroid Retinoid binding protein Cell Biology Retinoic acid receptor gamma eye diseases Retinoic acid receptor 030104 developmental biology Gene Expression Regulation chemistry Retinoic acid receptor alpha biology.protein sense organs Chickens |
| Zdroj: | Journal of Biological Chemistry. 291:18991-19005 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m116.725523 |
| Popis: | All-trans-retinoic acid may be an important molecular signal in the postnatal control of eye size. The goal of this study was to identify retinoic acid-binding proteins secreted by the choroid and sclera during visually guided ocular growth. Following photoaffinity labeling with all-trans-[11,12-(3)H]retinoic acid, the most abundant labeled protein detected in the conditioned medium of choroid or sclera had an apparent Mr of 27,000 Da. Following purification and mass spectrometry, the Mr 27,000 band was identified as apolipoprotein A-I. Affinity capture of the radioactive Mr 27,000 band by anti-chick apolipoprotein A-I antibodies confirmed its identity as apolipoprotein A-I. Photoaffinity labeling and fluorescence quenching experiments demonstrated that binding of retinoic acid to apolipoprotein A-I is 1) concentration-dependent, 2) selective for all-trans-retinoic acid, and 3) requires the presence of apolipoprotein A-I-associated lipids for retinoid binding. Expression of apolipoprotein A-I mRNA and protein synthesis were markedly up-regulated in choroids of chick eyes during the recovery from induced myopia, and apolipoprotein A-I mRNA was significantly increased in choroids following retinoic acid treatment. Together, these data suggest that apolipoprotein A-I may participate in a regulatory feedback mechanism with retinoic acid to control the action of retinoic acid on ocular targets during postnatal ocular growth. |
| Databáze: | OpenAIRE |
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