Phenethyl Isothiocyanate Protects against High Fat/Cholesterol Diet-Induced Obesity and Atherosclerosis in C57BL/6 Mice

Autor: Young-Sun Im, Min-Hee Gwon, Kyoung Yun Kim, Ha-Rin Moon, A-Reum Seo, Jung-Mi Yun
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
obesity
Phenethyl isothiocyanate
CD36
Peroxisome proliferator-activated receptor
lcsh:TX341-641
Diet
High-Fat
Article
Cholesterol
Dietary
03 medical and health sciences
chemistry.chemical_compound
Mice
Random Allocation
0302 clinical medicine
phenethyl isothiocyanate
Isothiocyanates
Internal medicine
Hyperlipidemia
medicine
Animals
Scavenger receptor
Enzyme Inhibitors
chemistry.chemical_classification
Nutrition and Dietetics
Triglyceride
biology
Cholesterol
histone modifications
lipid accumulation
Reverse cholesterol transport
medicine.disease
Atherosclerosis
reverse cholesterol transport
Mice
Inbred C57BL
030104 developmental biology
Endocrinology
chemistry
inflammation
030220 oncology & carcinogenesis
biology.protein
lcsh:Nutrition. Foods and food supply
Food Science
Zdroj: Nutrients
Nutrients, Vol 12, Iss 3657, p 3657 (2020)
Volume 12
Issue 12
ISSN: 2072-6643
Popis: This study concerns obesity-related atherosclerosis, hyperlipidemia, and chronic inflammation. We studied the anti-obesity and anti-atherosclerosis effects of phenethyl isothiocyanate (PEITC) and explored their underlying mechanisms. We established an animal model of high fat/cholesterol-induced obesity in C57BL/6 mice fed for 13 weeks. We divided the mice into five groups: control (CON), high fat/cholesterol (HFCD), HFCD with 3 mg/kg/day gallic acid (HFCD + G), and HFCD with PEITC (30 and 75 mg/kg/day
HFCD + P30 and P75). The body weight, total cholesterol, and triglyceride were significantly lower in the HFCD + P75 group than in the HFCD group. Hepatic lipid accumulation and atherosclerotic plaque formation in the aorta were significantly lower in both HFCD + PEITC groups than in the HFCD group, as revealed by hematoxylin and eosin (H&
E) staining. To elucidate the mechanism, we identified the expression of genes related to inflammation, reverse cholesterol transport, and lipid accumulation pathway in the liver. The expression levels of peroxisome proliferator activated receptor gamma (PPAR&gamma
), liver-X-receptor &alpha
(LXR-&alpha
), and ATP binding cassette subfamily A member 1 (ABCA1) were increased, while those of scavenger receptor A (SR-A1), cluster of differentiation 36 (CD36), and nuclear factor-kappa B (NF-&kappa
B) were decreased in the HFCD + P75 group compared with those in the HFCD group. Moreover, PEITC modulated H3K9 and H3K27 acetylation, H3K4 dimethylation, and H3K27 di-/trimethylation in the HFCD + P75 group. We, therefore, suggest that supplementation with PEITC may be a potential candidate for the treatment and prevention of atherosclerosis and obesity.
Databáze: OpenAIRE
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