An efficient cumate-inducible system for procyclic and bloodstream form Trypanosoma brucei
| Autor: | Htay Mon Aye, Kevin S. W. Tan, Cynthia Y. He, Zhao-Rong Lun, Zhi-Shen Xu, Feng-Jun Li, Anaïs Brasseur |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transcriptional Activation Gfp reporter Trypanosoma brucei brucei RNA Biology Trypanosoma brucei biology.organism_classification Molecular biology Benzoates Green fluorescent protein Cell biology law.invention 03 medical and health sciences 030104 developmental biology law parasitic diseases Gene expression Recombinant DNA Parasitology Gene Molecular Biology |
| Zdroj: | Molecular and biochemical parasitology. 214 |
| ISSN: | 1872-9428 |
| Popis: | In Trypanosoma brucei, the tetracycline-inducible system enables tightly-regulated, highly-efficient expression of recombinant proteins or double-stranded RNA in both procyclic and bloodstream form cells, providing useful molecular genetic tools to study gene functions. An alternative, vanillic acid-inducible system is recently described for procyclic T. brucei, providing ∼18-fold increase in GFP reporter expression upon induction (Sunter JD. Mol. Biochem. Parasitol. 2016, 207:45-48). Here we describe a cumate-inducible system that allows efficient, tunable gene expression showing >300-fold increase in GFP expression upon induction. The cumate-inducible system can be used alone or together with the tetracycline-inducible system, in both procyclic and bloodstream form T. brucei. Efficient cumate-inducible expression is also achieved in T. brucei-infected mice. |
| Databáze: | OpenAIRE |
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