Chitosan/tripolyphosphate nanoformulation carrying paraquat: insights on its enhanced herbicidal activity
| Autor: | Mariana M.L. Forini, Etenaldo Felipe Santiago, Renato Grillo, Montcharles da Silva Pontes, Anderson R.L. Caires, Debora Ribeiro Antunes, Gilberto J. Arruda, Jaqueline da Silva Santos, Ivan Pires de Oliveira |
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| Přispěvatelé: | Universidade Estadual de Mato Grosso do Sul (UEMS), Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), Universidade Federal de Mato Grosso do Sul (UFMS), University of Essex Colchester |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
P700 Materials Science (miscellaneous) medicine.medical_treatment 02 engineering and technology 010501 environmental sciences 021001 nanoscience & nanotechnology Ligand (biochemistry) Photosystem I 01 natural sciences Redox Chitosan Lipid peroxidation chemistry.chemical_compound Paraquat chemistry medicine Biophysics 0210 nano-technology 0105 earth and related environmental sciences General Environmental Science |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 2051-8161 2051-8153 |
| DOI: | 10.1039/d0en01128b |
| Popis: | Made available in DSpace on 2021-06-25T10:31:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-21 Despite the wide range of possible applications of nanopesticides, the mechanisms involved in their enhanced action remain largely unknown. Understanding the interaction between nanopesticides and plants is crucial for evaluating their potential safety application. Using an experimental and theoretical approach, this study aimed to investigate the target effect of paraquat-loaded chitosan/tripolyphosphate nanoparticles (ca. 200 nm) on photosystem I (PSI). Electrochemical analysis showed distinct electroactivity behaviour between the encapsulated and non-encapsulated herbicide. The amount of lipid peroxidation, photooxidizable P700 reaction centre content, and NADPH/NADP+ ratio levels were significantly decreased in spinach leaf tissue exposed to the nanoherbicide compared to those with the non-encapsulated herbicide. The data also revealed that the nanoformulation might promote oxidative stress based on changes observed in antioxidant enzymes. Also, molecular docking results showed a preferential disposition of the herbicide paraquat and paraquat-tripolyphosphate complex (TPP:PQ) in the ligand domain close to FAD and Glu312. Due to the inhibitor's strategic position in the catalytic pocket, a model of electron-capture is proposed, where the herbicide disturbs the redox process NADP+ ⇌ NADPH by capturing electrons to reduce itself. Finally, our findings provide important insights where the redox behaviour of paraquat may play a key role in the enhanced efficiency of nanoherbicides to the target binding site. Plant Resources Study Group Natural Resources Program Center for Natural Resources Study (CERNA) Mato Grosso Do sul State University (UEMS) São Paulo State University (UNESP) Department of Physics and Chemistry Faculty of Engineering Department of Pharmacology Institute of Biomedical Sciences University of São Paulo (USP) Optics and Photonics Group Institute of Physics Federal University of Mato Grosso Do sul (UFMS) School of Life Science University of Essex Colchester São Paulo State University (UNESP) Department of Physics and Chemistry Faculty of Engineering |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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