A differential requirement for phosphoinositide 3-kinase reveals two pathways for inducible upregulation of major histocompatibility complex class II molecules and CD86 expression by murine B lymphocytes
| Autor: | Stuart Marshall-Clarke, Lynn Tasker, R. Michael E. Parkhouse, Mark P Heaton |
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| Rok vydání: | 2003 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD74 Morpholines Immunology Antigen presentation Biology Major histocompatibility complex Mice Phosphatidylinositol 3-Kinases Antigen Antigens CD MHC class I Animals Immunology and Allergy Lectins C-Type Enzyme Inhibitors Cells Cultured Phosphoinositide-3 Kinase Inhibitors CD86 B-Lymphocytes Mice Inbred BALB C Membrane Glycoproteins Antigen processing Histocompatibility Antigens Class II Original Articles MHC restriction Molecular biology Up-Regulation Chromones biology.protein Female B7-2 Antigen Cell Division |
| Zdroj: | Immunology. 109:102-108 |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | Constitutive expression of major histocompatibility complex class II molecules (MHC II) is restricted to dendritic cells, cells of the macrophage lineage and B lymphocytes. In all three lineages, peptide fragments of captured antigen are loaded into newly synthesized MHC II molecules. In B-lineage cells, MHC II synthesis is dramatically increased on encounter with antigen, by T-cell-derived signals and by microbial products. We have previously shown that immature B cells fail to hyperexpress MHC II after antigen receptor [B-cell receptor (BCR)] ligation, but are responsive to other stimuli. Expression of the costimulatory molecule, CD86, was similarly regulated. This suggested the existence of two pathways regulating expression of these important molecules. Here we present data supporting this hypothesis. We show that activity of the enzyme phosphatidylinositol 3-kinase is critical for MHC II hyperexpression and induction of CD86 in response to ligation of the BCR or CD38, but not for responses to other stimuli including interleukin-4, lipopolysaccharide and CD40 ligation. |
| Databáze: | OpenAIRE |
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