Translational Genomics in Legumes Allowed Placing In Silico 5460 Unigenes on the Pea Functional Map and Identified Candidate Genes in Pisum sativum L
| Autor: | Florent Murat, Hervé Houtin, Vincent Savois, Jean Potier, Grégoire Aubert, Judith Burstin, Marie Georgette Nicolas, Amandine Bordat, Michael Bourgeois, Aurélie Chauveau, Céline Rond, Pascal Marget, Jérôme Salse |
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| Přispěvatelé: | Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), FP6 EU project 'Grain Legumes Integrated Project' FOOD-CT-2004-506223, GLIP, French national programs Genoplante GOP-PEAC2, GOP-PEAC2, Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
translational genomics [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Candidate gene Genome evolution functional consensus map In silico translational genomic Biology CARTOGRAPHIE GENETIQUE 01 natural sciences Genome 03 medical and health sciences Genetics Molecular Biology Gene Genetics (clinical) pisum sativum 030304 developmental biology Synteny model legume specie 2. Zero hunger Investigation 0303 health sciences fungi synteny food and beverages model legume species Phenotypic trait Genetic marker 010606 plant biology & botany |
| Zdroj: | G3 G3, Genetics Society of America, 2011, 1 (juillet), pp.93-103. ⟨10.1534/g3.111.000349⟩ G3, 2011, 1 (juillet), pp.93-103. ⟨10.1534/g3.111.000349⟩ G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | To identify genes involved in phenotypic traits, translational genomics from highly characterized model plants to poorly characterized crop plants provides a valuable source of markers to saturate a zone of interest as well as functionally characterized candidate genes. In this paper, an integrated view of the pea genetic map was developed. A series of gene markers were mapped and their best reciprocal homologs were identified on M. truncatula, L. japonicus, soybean, and poplar pseudomolecules. Based on the syntenic relationships uncovered between pea and M. truncatula, 5460 pea Unigenes were tentatively placed on the consensus map. A new bioinformatics tool, http://www.thelegumeportal.net/pea_mtr_translational_toolkit, was developed that allows, for any gene sequence, to search its putative position on the pea consensus map and hence to search for candidate genes among neighboring Unigenes. As an example, a promising candidate gene for the hypernodulation mutation nod3 in pea was proposed based on the map position of the likely homolog of Pub1, a M. truncatula gene involved in nodulation regulation. A broader view of pea genome evolution was obtained by revealing syntenic relationships between pea and sequenced genomes. Blocks of synteny were identified which gave new insights into the evolution of chromosome structure in Papillionoids and Eudicots. The power of the translational genomics approach was underlined. |
| Databáze: | OpenAIRE |
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