Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin
| Autor: | Mohamed Nejib Marzouki, Hayet Mhamdi, Nehla Mokni-Baizig, Sonia M'hirsi, Nihel Ammous-Boukhris, Said Gritli, Saoussen Debouki-Joudi, Raja Mokdad Gargouri |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Cancer Research Article Subject Adolescent DNA Copy Number Variations Nasopharyngeal neoplasm Biology Pathology and Forensic Medicine Metastasis Young Adult 03 medical and health sciences 0302 clinical medicine Cyclin D1 Antigens CD Kruppel-Like Factor 6 Carcinoma medicine Humans RC254-282 Aged Aged 80 and over Nasopharyngeal Carcinoma QH573-671 Cadherin Neoplasms. Tumors. Oncology. Including cancer and carcinogens Nasopharyngeal Neoplasms Cell Biology General Medicine Middle Aged Cadherins medicine.disease Alternative Splicing 030104 developmental biology KLF6 Nasopharyngeal carcinoma Tumor progression 030220 oncology & carcinogenesis Cancer research Molecular Medicine Female Cytology Research Article |
| Zdroj: | Analytical Cellular Pathology, Vol 2018 (2018) Analytical Cellular Pathology (Amsterdam) |
| ISSN: | 2210-7185 2210-7177 |
| DOI: | 10.1155/2018/9654067 |
| Popis: | Purpose. The transcription factor Krüppel-like factor 6 (KLF6) regulates various cellular functions, such as metabolism, cell proliferation, and differentiation. KLF6 plays a key role in the development and progression of multiple human cancers. Methods. Fifty primary biopsies and 10 normal nasopharyngeal mucosae were used to analyze by RT-QPCR the expression and the copy number of wtKLF6 and the spliced variants (KLF6-SV1, KLF6-SV2, and KLF6-SV3) in Tunisian patients with nasopharyngeal carcinoma. The expression analysis of E-cadherin and cyclin D1 was conducted by RT-QPCR and Western blot, respectively. Results. The wtKLF6 was significantly downexpressed in tumors compared to normal tissues (p=0.0015), whereas KLF6-SV1 and KLF6-SV2 were overexpressed in tumors compared to wtKLF6 and KLF6-SV3 (p<0.0001). Copy number variation was reduced in tumors compared to normal tissues (p=0.0071). Interestingly, KLF6-SV1 is associated with the juvenile form (p=0.0003) which is more aggressive than the adult form of NPC. Furthermore, the oncogenic variant KLF6-SV1 was overexpressed in tumors lacking the expression of E-cadherin (p=0.0022) suggesting its role in metastasis and tumor progression. The wtKLF6 is associated negatively with cyclin D1 in tumor tissues (p=0.048). Conclusion. The wtKLF6 was downexpressed in contrast with the oncogenic variants. Overexpression of KLF6-SV1 is associated with young patients, and loss of E-cadherin suggests that this variant correlated with the aggressiveness of NPC. |
| Databáze: | OpenAIRE |
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