An Ex Vivo Porcine Nasal Mucosa Explants Model to Study MRSA Colonization

Autor: Tulinski, P., Fluit, A.C., van Putten, J.P.M., de Bruin, A., Glorieux, S., Wagenaar, J.A., Duim, B., Advances in Veterinary Medicine, Strategic Infection Biology, Tissue Repair, Dep Infectieziekten Immunologie, Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), I&I SIB2, Dep Pathobiologie
Jazyk: angličtina
Rok vydání: 2013
Předmět:
BACTERIAL
METHICILLIN-RESISTANT
Swine
Applied Microbiology
Veterinary Microbiology
lcsh:Medicine
Mucous membrane of nose
Pathogenesis
medicine.disease_cause
CULTURE
PIGS
Colonization
lcsh:Science
Staphylococci
Multidisciplinary
Bacteriologie
Microbial Growth and Development
RESISTANT STAPHYLOCOCCUS-AUREUS
HUMANS
Bacteriology
Host Pathogen Interaction & Diagnostics
Staphylococcal Infections
Bacterial Pathogens
Host-Pathogen Interaction
INTERFACE
medicine.anatomical_structure
Medical Microbiology
Staphylococcus aureus
CARRIAGE
Medicine
Infectious diseases
Research Article
Methicillin-Resistant Staphylococcus aureus
Animal Types
Bacterial diseases
Nose
Large Animals
Biology
Staphylococcal infections
Microbiology
ADHERENCE
medicine
Animals
Life Science
Veterinary Sciences
Microbial Pathogens
Host Pathogen Interaction & Diagnostics
lcsh:R
Bacteriology
medicine.disease
Methicillin-resistant Staphylococcus aureus
Epithelium
Host Pathogen Interactie & Diagnostiek
Nasal Mucosa
MICE
Emerging Infectious Diseases
Bacteriologie
Host Pathogen Interactie & Diagnostiek
Veterinary Science
lcsh:Q
Ex vivo
Respiratory tract
Zdroj: PLoS One, 8(1). Public Library of Science
PLoS ONE, Vol 8, Iss 1, p e53783 (2013)
PLoS ONE 8 (2013) 1
PLoS ONE, 8(1)
PLOS ONE
PLoS ONE
ISSN: 1932-6203
Popis: Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa explants cultured at an air-liquid interface was evaluated. In cultured mucosa explants from the surfaces of the ventral turbinates and septum of the pig nose no changes in cell morphology and viability were observed up to 72 h. MRSA colonization on the explants was evaluated followed for three MRSA ST398 isolates for 180 minutes. The explants were incubated with 3×10(8) CFU/ml in PBS for 2 h to allow bacteria to adhere to the explants surface. Next the explants were washed and in the first 30 minutes post adhering time, a decline in the number of CFU was observed for all MRSA. Subsequently, the isolates showed either: bacterial growth, no growth, or a further reduction in bacterial numbers. The MRSA were either localized as clusters between the cilia or as single bacteria on the cilia surface. No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcine nasal mucosa explants are a valuable ex vivo model to unravel the interaction of MRSA with nasal tissue.
Databáze: OpenAIRE
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