Molecular characterization of irish salmonella enterica serotype typhimurium: detection of class i integrons and assessment of genetic relationships by dna amplification fingerprinting

Autor: Colette O'Hare, E. Power, Martin Cormican, Séamus Fanning, Patrick Wall, James F. Buckley, B. Cryan, M. Daly
Rok vydání: 2000
Předmět:
Salmonella typhimurium
Serotype
plasmids
Tetracycline
Molecular Sequence Data
Computational biology
Microbial Sensitivity Tests
Drug resistance
Characterization (mathematics)
Integron
Applied Microbiology and Biotechnology
insertion
Microbiology
Antibiotic resistance
Plasmid
Irish
medicine
dt104
Environmental Microbiology and Biodegradation
Animals
Humans
Bacteriophage Typing
Phage typing
Salmonella Infections
Animal
Ecology
biology
Errata
antibiotic-resistance genes
biochemical phenomena
metabolism
and nutrition
Dna amplification
bacterial infections and mycoses
biology.organism_classification
Class (biology)
DNA Fingerprinting
language.human_language
Anti-Bacterial Agents
Bacterial Typing Techniques
dt-104
Salmonella enterica
Salmonella Infections
language
DNA Transposable Elements
Food Microbiology
biology.protein
epidemiology
Cattle
Food Science
Biotechnology
medicine.drug
Popis: Salmonella enterica is among the principal etiological agents of food-borne illness in humans. Increasing antimicrobial resistance in S. enterica is a cause for worldwide concern. There is concern at present in relation to the increasing incidence of human infection with antimicrobial agent-resistant strains of S. enterica serotype Typhimurium, in particular of phage type DT104. Integrons appear to play an important role in the dissemination of antimicrobial resistance genes in many Enterobacteriaceae including S. enterica . In this study the antimicrobial susceptibilities and phage types of 74 randomly collected strains of S. enterica serotype Typhimurium from the Cork region of southern Ireland, obtained from human, animal (clinical), and food sources, were determined. Each strain was examined for integrons and typed by DNA amplification fingerprinting (DAF). Phage type DT104 predominated ( n = 48). Phage types DT104b ( n = 3), -193 ( n = 9), -195 ( n = 6), -208 ( n = 3), -204a ( n = 2), PT U302 ( n = 1), and two nontypeable strains accounted for the remainder. All S. enterica serotype Typhimurium DT104 strains were resistant to ampicillin, chloramphenicol, streptomycin, Sulfonamide Duplex, and tetracycline, and one strain was additionally resistant to trimethoprim. All DT104 strains but one were of a uniform DAF type (designated DAF-I) and showed a uniform pattern of integrons (designated IP-I). The DT104b and PT U302 strains also exhibited the same resistance phenotype, and both had the DAF-I and IP-I patterns. The DAF-I pattern was also observed in a single DT193 strain in which no integrons were detectable. Greater diversity of antibiograms and DAF and IP patterns among non-DT104 phage types was observed. These data indicate a remarkable degree of homogeneity at a molecular level among contemporary isolates of S. enterica serotype Typhimurium DT104 from animal, human, and food sources in this region.
Databáze: OpenAIRE
Externí odkaz: