Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells
| Autor: | Rafael Calixto Bortolin, Francilene Amaral da Silva, Grace Anne Azevedo Dória, José Cláudio Fonseca Moreira, Bruno S. Vasconcelos, Rebeca Oliveira de Souza, Paula dos Passos Menezes, Daniel Pens Gelain, Anderson Ribeiro dos Santos, Ana Paula do Nascimento Prata, Leonardo da Silva Bittencourt, Alan D. C. Santos, Adriano Antunes de Souza Araújo, Lucindo José Quintans-Júnior, Maria José Vieira Fonseca, Shanmugam Saravanan |
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| Rok vydání: | 2015 |
| Předmět: |
Neutral red
Antioxidant medicine.medical_treatment Melanoma Experimental Pharmaceutical Science free radicals Biology medicine.disease_cause Article Cell Line Analytical Chemistry lcsh:QD241-441 Lipid peroxidation Mice chemistry.chemical_compound Tar (tobacco residue) lcsh:Organic chemistry Cell Line Tumor Drug Discovery medicine Animals oxidative stress Physical and Theoretical Chemistry Cytotoxicity total phenol Plant Extracts Organic Chemistry Remirea maritima Biological activity Fibroblasts Antineoplastic Agents Phytogenic In vitro Biochemistry chemistry Chemistry (miscellaneous) cytotoxicity Molecular Medicine Cyperaceae Oxidation-Reduction Oxidative stress cellular viability |
| Zdroj: | Molecules, Vol 20, Iss 7, Pp 11699-11718 (2015) Molecules Volume 20 Issue 7 Pages 11699-11718 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules200711699 |
| Popis: | Remirea maritima is a tropical plant with a reticulated root system belonging to the family Cyperaceae, also known to have biologically active secondary metabolites. However, very few data on R. maritima’s biological actions are available and there are no reports regarding the redox-active profile of this plant. In this study, we examined the total phenolic content of Remirea maritima hydroalcoholic (RMHA) extracts, redox properties against different reactive species generated in vitro and their cytotoxic effect against fibroblasts (L929) and melanoma (B16F10) cells. Total reactive antioxidant potential index (TRAP) and total antioxidant reactivity (TAR) results revealed that RMHA at all concentrations tested showed significant antioxidant capacity. RMHA was also effective against hydroxyl radical formation, reduction of Fe3+ to Fe2+ and in scavenging nitric oxide (NO) radicals. In vitro, the level of lipid peroxidation was reduced by RMHA extract and the data showed significant oxidative damage protection. The RMHA cytotoxicity was evaluated by a neutral red assay in fibroblast (L929) and melanome (B16F10) cells. The obtained results showed that the RMHA (40 and 80 µg/mL, respectively) reduced 70% of the viable cells. In conclusion, this study represents the first report regarding the antioxidant and anti-proliferative potential of R. maritima against B16F10 melanoma cells. |
| Databáze: | OpenAIRE |
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