Altered phosphorylation, electrophysiology, and behavior on attenuation of PDE4B action in hippocampus
| Autor: | Thomas van Groen, Susan Campbell, Inga Kadish, Graeme B. Bolger, Lisa High Mitchell Smoot |
|---|---|
| Přispěvatelé: | Animal and Poultry Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Long-Term Potentiation Hippocampus Hippocampal formation Anxiety Synaptic Transmission 0302 clinical medicine Conditioning Psychological PKA Phosphorylation Cyclic AMP Response Element-Binding Protein Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 biology Depression CREB General Neuroscience Neurogenesis lcsh:QP351-495 Long-term potentiation Fear Isoenzymes Female Research Article PDE4B1 Transgene Mice Transgenic Motor Activity lcsh:RC321-571 03 medical and health sciences Cellular and Molecular Neuroscience DISC1 Memory Animals Learning lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Association Learning Cyclic Nucleotide Phosphodiesterases Type 4 030104 developmental biology lcsh:Neurophysiology and neuropsychology Synaptic plasticity Mutation biology.protein PDE4 Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | BMC Neuroscience, Vol 18, Iss 1, Pp 1-20 (2017) BMC Neuroscience |
| ISSN: | 1471-2202 |
| DOI: | 10.1186/s12868-017-0396-6 |
| Popis: | Background: PDE4 cyclic nucleotide phosphodiesterases regulate 3′, 5′ cAMP abundance in the CNS and thereby regulate PKA activity and phosphorylation of CREB, which has been implicated in learning and memory, depression and other functions. The PDE4 isoform PDE4B1 also interacts with the DISC1 protein, implicated in neural development and behavioral disorders. The cellular functions of PDE4B1 have been investigated extensively, but its function(s) in the intact organism remained unexplored. Results: To specifically disrupt PDE4B1, we developed mice that express a PDE4B1-D564A transgene in the hippocampus and forebrain. The transgenic mice showed enhanced phosphorylation of CREB and ERK1/2 in hippocampus. Hippocampal neurogenesis was increased in the transgenic mice. Hippocampal electrophysiological studies showed increased baseline synaptic transmission and enhanced LTP in male transgenic mice. Behaviorally, male transgenic mice showed increased activity in prolonged open field testing, but neither male nor female transgenic mice showed detectable anxiety-like behavior or antidepressant effects in the elevated plus-maze, tail-suspension or forced-swim tests. Neither sex showed any significant differences in associative fear conditioning or showed any demonstrable abnormalities in pre-pulse inhibition. Conclusions: These data support the use of an isoform-selective approach to the study of PDE4B1 function in the CNS and suggest a probable role of PDE4B1 in synaptic plasticity and behavior. They also provide additional rationale and a refined approach to the development of small-molecule PDE4B1-selective inhibitors, which have potential functions in disorders of cognition, memory, mood and affect. The McKnight Foundation (no grant number, for research support and also funding of the behavioral and electrophysiology cores), the Bolger Prostate Cancer Research Fund (no grant number), and the National Cancer Institute of the National Institutes of Health to the University of Alabama at Birmingham Comprehensive Cancer Center under award number P30 CA013148 (for generation of transgenic mice and DNA sequencing). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or the McKnight Foundation, neither of which played any role on the in study design; in the collection, analysis, and interpretation of data; in the writing of the manuscript; or in the decision to submit the manuscript for publication. Published version |
| Databáze: | OpenAIRE |
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