Molecular diversity and phylogeny of indigenous Rhizobium leguminosarum strains associated with Trifolium repens plants in Romania
| Autor: | Nikolaos E. Labrou, Culita Sirbu, Lucian Gorgan, Emmanouil Flemetakis, Craita Maria Rosu, Andrei Stefan, Rodica C. Efrose, Catalina Stedel |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology Biology medicine.disease_cause Microbiology Rhizobium leguminosarum Rhizobia 03 medical and health sciences Phylogenetics Botany medicine Molecular Biology Phylogeny Genes Essential Phylogenetic tree food and beverages Genetic Variation General Medicine Biodiversity Genomics biology.organism_classification Housekeeping gene Molecular Typing Phylogenetic diversity 030104 developmental biology Phenotype Genes Bacterial Trifolium repens Nitrogen fixation Trifolium Genome Bacterial Multilocus Sequence Typing |
| Zdroj: | Antonie van Leeuwenhoek. 111(1) |
| ISSN: | 1572-9699 |
| Popis: | The symbiotic nitrogen fixing legumes play an essential role in sustainable agriculture. White clover (Trifolium repens L.) is one of the most valuable perennial legumes in pastures and meadows of temperate regions. Despite its great agriculture and economic importance, there is no detailed available information on phylogenetic assignation and characterization of rhizobia associated with native white clover plants in South-Eastern Europe. In the present work, the diversity of indigenous white clover rhizobia originating in 11 different natural ecosystems in North-Eastern Romania were assessed by a polyphasic approach. Initial grouping showed that, 73 rhizobial isolates, representing seven distinct phenons were distributed into 12 genotypes, indicating a wide phenotypic and genotypic diversity among the isolates. To clarify their phylogeny, 44 representative strains were used in sequence analysis of 16S rRNA gene and IGS fragments, three housekeeping genes (atpD, glnII and recA) and two symbiosis-related genes (nodA and nifH). Multilocus sequence analysis (MLSA) phylogeny based on concatenated housekeeping genes delineated the clover isolates into five putative genospecies. Despite their diverse chromosomal backgrounds, test strains shared highly similar symbiotic genes closely related to Rhizobium leguminosarum biovar trifolii. Phylogenies inferred from housekeeping genes were incongruent with those of symbiotic genes, probably due to occurrence of lateral transfer events among native strains. This is the first polyphasic taxonomic study to report on the MLSA-based phylogenetic diversity of indigenous rhizobia nodulating white clover plants grown in various soil types in South-Eastern Europe. Our results provide valuable taxonomic data on native clover rhizobia and may increase the pool of genetic material to be used as biofertilizers. |
| Databáze: | OpenAIRE |
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