Non-marking Collection of Amino Acids from Fingerprints Using Hydrogels

Autor: van Helmond, Ward, O’Brien, Vincent, de Jong, Robin, van Esch, Jan, Oldenhof, Sander, de Puit, Marcel, Alterman, Michail A.
Přispěvatelé: Lectoraat Forensisch Onderzoek
Rok vydání: 2019
Předmět:
Zdroj: Methods in Molecular Biology ISBN: 9781493996384
Amino acid analysis: methods and protocols, 429-438
ISSUE=2;STARTPAGE=429;ENDPAGE=438;TITLE=Amino acid analysis
DOI: 10.1007/978-1-4939-9639-1_32
Popis: The amino acid profile obtained from a fingerprint may provide valuable information on its donor. Unfortunately, the collection of chemicals from the fingerprint is often destructive to the fingerprint ridge detail. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualization at a later stage using the standard method of cyanoacrylate fuming followed by basic-yellow dyeing.
Databáze: OpenAIRE