Baker’s Yeast Deficient in Storage Lipid Synthesis Uses cis-Vaccenic Acid to Reduce Unsaturated Fatty Acid Toxicity
| Autor: | Milan Čertík, Peter Gajdoš, Martina Garaiova, Roman Holic, Ivan Hapala, Peter Griac, Peter Sec |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Growth medium Saccharomyces cerevisiae Proteins Organic Chemistry Fatty acid Vaccenic acid Oleic Acids Saccharomyces cerevisiae Cell Biology Biology Biochemistry Yeast Culture Media Fatty Acids Monounsaturated Oleic acid chemistry.chemical_compound chemistry Mutation Palmitoleic acid Diacylglycerol O-Acyltransferase Triglycerides Unsaturated fatty acid Sterol O-Acyltransferase Polyunsaturated fatty acid |
| Zdroj: | Lipids. 50:621-630 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/s11745-015-4022-z |
| Popis: | The role of cis-vaccenic acid (18:1n-7) in the reduction of unsaturated fatty acids toxicity was investigated in baker's yeast Saccharomyces cerevisiae. The quadruple mutant (QM, dga1Δ lro1Δ are1Δ are2Δ) deficient in enzymes responsible for triacylglycerol and steryl ester synthesis has been previously shown to be highly sensitive to exogenous unsaturated fatty acids. We have found that cis-vaccenic acid accumulated during cultivation in the QM cells but not in the corresponding wild type strain. This accumulation was accompanied by a reduction in palmitoleic acid (16:1n-7) content in the QM cells that is consistent with the proposed formation of cis-vaccenic acid by elongation of palmitoleic acid. Fatty acid analysis of individual lipid classes from the QM strain revealed that cis-vaccenic acid was highly enriched in the free fatty acid pool. Furthermore, production of cis-vaccenic acid was arrested if the mechanism of fatty acids release to the medium was activated. We also showed that exogenous cis-vaccenic acid did not affect viability of the QM strain at concentrations toxic for palmitoleic or oleic acids. Moreover, addition of cis-vaccenic acid to the growth medium provided partial protection against the lipotoxic effects of exogenous oleic acid. Transformation of palmitoleic acid to cis-vaccenic acid is thus a rescue mechanism enabling S. cerevisiae cells to survive in the absence of triacylglycerol synthesis as the major mechanism for unsaturated fatty acid detoxification. |
| Databáze: | OpenAIRE |
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