Intra-arterial Instillation of a Nociceptive Agent Modulates Cardiorespiratory Parameters Involving 5-HT3 and TRPV1 Receptors in Anesthetized Rats
| Autor: | Madaswamy S Muthu, Ravindran Revand, Sanjeev K. Singh, Maloy B Mandal |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Agonist
Male Nociception medicine.drug_class Vasodilator Agents TRPV1 Bradykinin TRPV Cation Channels chemistry.chemical_compound Reflex medicine Animals Pharmacology business.industry Respiration Antagonist Hematology General Medicine Rats medicine.anatomical_structure chemistry Anesthesia Molecular Medicine Hypotension Receptors Serotonin 5-HT3 Cardiology and Cardiovascular Medicine Capsazepine business Sensory nerve |
| Zdroj: | Cardiovascularhematological disorders drug targets. 21(1) |
| ISSN: | 2212-4063 |
| Popis: | Background: Since long back, it has been a matter of discussion regarding the role of peripheral blood vessels in the regulation of cardiorespiratory (CVR) system. Objective: The role of 5-HT3 and TRPV1 receptors present on perivascular nerves in elicitation of CVR reflexes was examined after intra-arterial instillation of bradykinin in urethane anesthetized rats. Materials and Methods: Femoral artery was cannulated retrogradely and was utilized for the instillation of saline/agonist/antagonist and recording of blood pressure (BP), using a double ported 24G cannula. BP, respiration and ECG were recorded for 30 min after bradykinin (1 μM) in the absence or presence of antagonists. Results: Instillation of bradykinin produced immediate hypotensive (40%), bradycardiac (17%), tachypnoeic (45%) and hyperventilatory (96%) responses of shorter latencies (5-8 s) favoring the neural mechanisms in producing the responses. In lignocaine (2%) pretreated animals, bradykinin- induced hypotensive (10%), bradycardiac (1.7%), tachypnoeic (13%) and hyperventilatory (13%) responses attenuated significantly. Pretreatment with ondansetron (100 μg/kg), 5-HT3-antagonist attenuated the hypotensive (10%), bradycardiac (1.7%), tachypnoeic (11%) and hyperventilatory (11%) responses significantly. Pretreatment with capsazepine (1 mg/kg), transient receptor potential vanilloid 1- antagonist blocked the hypotensive (5%), bradycardiac (1.2%), tachypnoeic (6%) and hyperventilatory (6%) responses significantly. Conclusion: In conclusion, presence of a nociceptive agent in the local segment of an artery evokes vasosensory reflex responses modulating CVR parameters involving TRPV1 and 5-HT3 receptors present on the perivascular sensory nerve terminals in anesthetized rats. |
| Databáze: | OpenAIRE |
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