Promoter methylation of WNT inhibitory factor-1 and expression pattern of WNT/β-catenin pathway in human astrocytoma: pathologic and prognostic correlations
| Autor: | Seong Who Kim, Shin Kwang Khang, Hyang Ju Lee, Jihye Kwak, Hae Yun Nam, Sun A Kim, Sung-Min Chun, Byoung Wook Lee |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Adolescent Fluorescent Antibody Technique Astrocytoma WIF1 Biology Pathology and Forensic Medicine Young Adult Cyclin D1 medicine Humans Child Promoter Regions Genetic Wnt Signaling Pathway Adaptor Proteins Signal Transducing Aged Regulation of gene expression Brain Neoplasms Reverse Transcriptase Polymerase Chain Reaction Wnt signaling pathway Methylation DNA Methylation Middle Aged medicine.disease Immunohistochemistry Molecular biology Gene Expression Regulation Neoplastic Repressor Proteins Isocitrate dehydrogenase Tissue Array Analysis Catenin Cancer research Female Neoplasm Grading |
| Zdroj: | Modern Pathology. 26:626-639 |
| ISSN: | 0893-3952 |
| DOI: | 10.1038/modpathol.2012.215 |
| Popis: | WNT inhibitory factor-1 (WIF1) is an antagonist of the WNT signaling pathway. We investigated the relationship between WIF1 promoter methylation and regulation of the WNT/β-catenin signaling pathway, tumor grade, and survival in patients with astrocytoma. This study included 86 cases of astrocytoma, comprising 20 diffuse astrocytomas and 66 glioblastomas. In addition, 17 temporal lobectomy specimens from patients with epilepsy were included as controls. The ratio of methylated DNA to total methylated and unmethylated DNA (% methylation) was measured by methylation- and unmethylation-specific PCR. Representative tumor tissue was immunostained for WIF1, β-catenin, cyclin D1, c-myc, and isocitrate dehydrogenase 1. Levels of WIF1 promoter methylation, mRNA expression, and protein expression in a glioblastoma cell line were compared before and after demethylation treatment. The mean percent methylation of the WIF1 promoter in astrocytomas was higher than that in control brain tissue. WIF1 protein expression was lower in the tumor group with >5% methylation than in the group with |
| Databáze: | OpenAIRE |
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