Simple quantitation for potential serum disease biomarker peptides, primarily identified by a peptidomics approach in the serum with hypertensive disorders of pregnancy
| Autor: | Yoshihiko Araki, Daisuke Nonaka, Michio Nojima, Kenji Takamori, Tanaka Kenji, Hitoshi Ishikawa, Koyo Yoshida, Lyang-Ja Lee, Michio Banzai, Kensuke Hamamura, Mitsuaki Yanagida, Satoru Takeda |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Proteomics 030213 general clinical medicine Pregnancy 030219 obstetrics & reproductive medicine Chemistry Clinical Biochemistry General Medicine Hypertension Pregnancy-Induced medicine.disease 03 medical and health sciences Young Adult 0302 clinical medicine Pre-Eclampsia Serum biomarkers Immunology medicine Disease biomarker Humans Female Peptides Biomarkers |
| Zdroj: | Annals of clinical biochemistry. 53(Pt 1) |
| ISSN: | 1758-1001 |
| Popis: | Background We previously reported peptide candidates of disease biomarkers for pregnancy-induced hypertension syndrome using a novel peptidomic analytical method, BLOTCHIP®-MS. The aim of this study was to establish a sandwich enzyme-linked immunosorbent assay system for quantitation of such peptides and to validate their usefulness as disease biomarkers of pregnancy-induced hypertension syndrome including gestational hypertension/pre-eclampsia. Methods We focused on three peptide fragments, kininogen-1439–456 (PDA039), kininogen-1438–456 (PDA044) and cysteinyl α2-HS-glycoprotein341–367 (PDA071). Using polyclonal antibodies specific for each peptide, suitable conditions for the sandwich enzyme-linked immunosorbent assay system were investigated. The quantitative enzyme-linked immunosorbent assay values were confirmed by quantitative matrix assisted laser desorption/ionization time-of-flight MS analyses. Using the established enzyme-linked immunosorbent assay systems, serum samples from gestational hypertension/pre-eclampsia patients and paired serum samples from healthy pregnant females were analysed. Results The optimum sandwich enzyme-linked immunosorbent assay conditions for PDA039/044 quantitation were developed. Quantitation of PDA071 by enzyme-linked immunosorbent assay failed, presumably due to issues with polyclonal antibody specificity for the native peptide. Bland–Altman plots showed a satisfactory correlation between the serum PDA039/044 concentration by enzyme-linked immunosorbent assay and that by quantitative MS analysis. Although the PDA044 concentration showed no significant change during pregnancy, including gestational hypertension/pre-eclampsia patients, the serum PDA039 concentration was significantly increased ( P Conclusions The simple quantitation technology for PDA039 by enzyme-linked immunosorbent assay was established for the first time. PDA039 confirmed its clinical utility as a disease biomarker for gestational hypertension/pre-eclampsia by the enzyme-linked immunosorbent assay system using clinical samples. The information provided from the present study would be a new valuable addition in the field of gestational hypertension/pre-eclampsia research. |
| Databáze: | OpenAIRE |
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