Curcumin restores hepatic epigenetic changes in propylthiouracil(PTU) Induced hypothyroid male rats: A study on DNMTs, MBDs, GADD45a, C/EBP-β and PCNA

Autor: Suresh Kumar Bunker, Jyotsnarani Pradhan, G. B. N. Chainy, Jagneshwar Dandapat, Abinash Dutta
Rok vydání: 2019
Předmět:
Male
endocrine system
Curcumin
Antioxidant
Side effect
medicine.medical_treatment
Population
Cell Cycle Proteins
Pharmacology
Toxicology
medicine.disease_cause
Epigenesis
Genetic
03 medical and health sciences
chemistry.chemical_compound
Curcuma
0404 agricultural biotechnology
Hypothyroidism
Proliferating Cell Nuclear Antigen
medicine
Animals
Humans
DNA (Cytosine-5-)-Methyltransferases
education
030304 developmental biology
0303 health sciences
education.field_of_study
Endodeoxyribonucleases
biology
CCAAT-Enhancer-Binding Protein-beta
Nuclear Proteins
04 agricultural and veterinary sciences
General Medicine
040401 food science
Rats
Proliferating cell nuclear antigen
Liver
chemistry
Propylthiouracil
Hepatic stellate cell
biology.protein
hormones
hormone substitutes
and hormone antagonists
Oxidative stress
Food Science
medicine.drug
Zdroj: Food and Chemical Toxicology. 123:169-180
ISSN: 0278-6915
Popis: 6-n-propyl-2-thiouracil (PTU), a thioamide drug, is used as an effective anti-thyroid agent to treat hyperthyroidism and Graves' disease. However, acute liver oxidative damage is an important side effect of the drug. In the present study, we report that PTU administration to rat induces hepatic epigenetic changes by upregulating expression of DNMT1, DNMT3a, DNMT3b, MBD4, MeCP2, p53 and Gadd45a and down-regulation of PCNA and C/EBP-β. This is accompanied by decrease in the cell population and augmentation of cellular lipid peroxidation, an index of oxidative stress, in liver. On the other hand, co-administration of curcumin, a polyphenol extract from the rhizome of Curcuma longa L, along with PTU ameliorates PTU- induced oxidative stress and epigenetic parameters except for the expression of MBD4. Also, co-administration of curcumin with PTU resulted in restoration of hepatic cell population and histoarchitecture. The protective effect of curcumin to PTU-induced hepatotoxicity is attributed to its antioxidative properties.
Databáze: OpenAIRE
Externí odkaz: