Xanthomonas albilineans OmpA1 appears to be functionally modular and both the OMC and C-like domains are necessary for leaf scald disease of sugarcane
| Autor: | Shujian Zhang, Philippe Rott, Daniel Gargani, Imène Mensi, Laura Fleites, Dean W. Gabriel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Xanthomonas Physiology Sequence analysis Pouvoir pathogène Mutant Mutagenesis (molecular biology technique) Xanthomonas albilineans Biology medicine.disease_cause Protéine microbienne Bacterial genetics Microbiology Microscopy Electron Transmission medicine Escherichia coli Coding region Plant Diseases H20 - Maladies des plantes Génie génétique Saccharum officinarum Cell Membrane Genetic Complementation Test General Medicine Sequence Analysis DNA Résistance aux maladies Protein Structure Tertiary Saccharum Plant Leaves Mutagenesis Insertional Phenotype Bacterial outer membrane Agronomy and Crop Science Bacterial Outer Membrane Proteins |
| Zdroj: | Molecular Plant-Microbe Interactions |
| Popis: | Several EZ-Tn5 insertions in gene locus XALc_0557 (OmpA1) of the sugarcane leaf scald pathogen Xanthomonas albilineans XaFL07-1 were previously found to strongly affect pathogenicity and endophytic stalk colonization. XALc_0557 has a predicted OmpA N-terminal outer membrane channel (OMC) domain and an OmpA C-like domain. Further analysis of mutant M468, with an EZ-Tn5 insertion in the upstream OMC domain coding region, revealed impaired epiphytic and endophytic leaf survival, impaired resistance to sodium dodecyl sulfate (SDS), structural defects in the outer membrane (OM), and hyperproduction of OM vesicles. Cloned full-length XALc_0557 complemented M468 for all phenotypes tested, including pathogenicity, resistance to SDS, and ability to survive both endophytically and epiphytically. Another construct, pCT47.3, which expressed only the C-like domain of XALc_0557, restored resistance to SDS in M468 but failed to complement any other mutant phenotype, indicating that the C-like domain functioned independently of the OMC domain to help maintain OM integrity. pCT47.3 also complemented pathogenicity, resistance to SDS, and stalk colonization in mutant M1152, which carries an EZ-Tn5 insert in the C-like coding region, indicating that both predicted domains are modular and necessary but neither is sufficient for X. albilineans pathogenicity, endophytic survival in, and epiphytic survival on sugarcane. |
| Databáze: | OpenAIRE |
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