HLA frequency in candidates to transplant without compatible cord blood at the National Center of Blood Transfusion (Mexico)
| Autor: | Julieta Rojo-Medina, Juan Manuel Bello-López |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Blood transfusion medicine.medical_treatment Cord Blood Stem Cell Transplantation Human leukocyte antigen Umbilical cord 03 medical and health sciences 0302 clinical medicine Gene Frequency HLA Antigens Internal medicine medicine Humans Mexico Genotyping business.industry Haplotype Genetic Variation Hematology Fetal Blood Transplantation 030104 developmental biology medicine.anatomical_structure Cord blood Immunology Female business 030215 immunology |
| Zdroj: | Transfusion and Apheresis Science. 56:571-575 |
| ISSN: | 1473-0502 |
| Popis: | Introduction Umbilical Cord Blood Units (UCBU) for transplantation, are a therapeutic possibility for patients with a wide range of oncohaematological diseases and other immunologic disorders. The search of compatible donors for bone marrow transplantation is increasingly difficult for patients of mixed ethnicity. The aim of this work was determine the HLA frequency of candidates for transplantation without compatible UCBU at the National Center of from Blood Transfusion (NCBT) – Mexico. Material and methods A retrospective analysis of candidates to transplant without compatible UCBU was performed in the archives from 2003 to 2016 at the NCBT. HLA class I and II genotyping of candidates was performed by medium resolution methods: Sequence Specific Primer and/or Specific Sequence Oligonucleotide. HLA frequencies were obtained by including individuals without any particular bias to a phenotype and strict statistical and genetic analysis of populations were done. The database in www.allelefrequencies.net was used in order to identify the ethnic origin of the most frequent alleles. Results Three hundred and sixty-four candidates without compatible UCBU for transplantation were identified. The most frequent haplotype HLA I and II were: HLA-A*02/02, 02/24, 24/24, 02/68, 01/24 and 24/68; HLA-B*39/39, 35/51, 44/44, 44/40, 35/40 and 35/35; HLA-DRB1*04/04, 13/07, 04/13, 13/13 and 03/11. The ethnic origins of the analyzed data were represented in most cases by Amerindians, Caucasics, Orientals, Asians, Arabs and Africans. Conclusion This work shows the existence of a broad genetic diversity of candidates for transplantation with UCBU, making it difficult to find compatible units considering donors only from the capital. |
| Databáze: | OpenAIRE |
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