Photoactivatable Sensors for Detecting Mobile Zinc
| Autor: | Nathan W. Vogler, Daniel Y. Zhang, Thanos Tzounopoulos, Fang Wang, Chanan D. Sessler, Stephen J. Lippard, Jacob M. Goldberg, William H. Loucks |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Ultraviolet Rays
Metal ions in aqueous solution chemistry.chemical_element Zinc 010402 general chemistry 01 natural sciences Biochemistry Catalysis Article Fluorescence Colloid and Surface Chemistry In vivo Humans Binding site Fluorescent Dyes Brain Chemistry 010405 organic chemistry Chemistry Time resolution General Chemistry 0104 chemical sciences Spectrometry Fluorescence Microscopy Fluorescence Biophysics HeLa Cells |
| Popis: | Fluorescent sensors for mobile zinc have proven valuable for studying complex biological systems. Because these sensors typically bind zinc rapidly and tightly, there has been little temporal control over the activity of the probe after its application to a sample. The ability to control the activity of a zinc sensor in vivo during imaging experiments would greatly improve the time resolution of the measurement. Here, we describe photoactivatable zinc sensors that can be triggered with short pulses of UV light. These probes are prepared by functionalizing a zinc sensor with protecting groups that render the probe insensitive to metal ions. Photo-induced removal of the protecting groups restores the binding site, allowing for zinc-responsive changes in fluorescence that can be observed in live cells and tissues. |
| Databáze: | OpenAIRE |
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