Use of Electrospray Mass Spectrometry for Mass Determination of Grape (Vitis vinifera) Juice Pathogenesis-Related Proteins: A Potential Tool for Varietal Differentiation
| Autor: | Yoji Hayasaka, Gayle A. Baldock, Kathryn S. Adams, K. F. Pocock, Elizabeth J. Waters, Peter B. Høj |
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| Přispěvatelé: | Hayasaka, Yoji, Adams, Kathryn S, Pocock, Kenneth F, Baldock, G A, Waters, Elizabeth Joy |
| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
Electrospray Chromatography biology food and beverages General Chemistry Mass spectrometry Pathogenesis Enzyme chemistry Liquid chromatography–mass spectrometry Thaumatin Chitinase biology.protein Food science General Agricultural and Biological Sciences Pathogenesis-related protein |
| Zdroj: | Journal of Agricultural and Food Chemistry. 49:1830-1839 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf001163+ |
| Popis: | Methods based on liquid chromatography−mass spectrometry (LC-MS) and protein trap mass spectrometry (trap-MS) were developed to determine the complement of pathogenesis-related (PR) proteins in grape juice. Trap-MS was superior to LC-MS in terms of simplicity, rapidity, and sensitivity. Proteins with a wide range of masses (13−33 kDa) were found in the juices of 19 different varieties of grape (Vitis vinifera) and were identified as mostly PR-5 type (thaumatin-like) and PR-3 type (chitinases) proteins. Although the PR proteins in juices of grapes are highly conserved, small consistent differences in molecular masses were noted when otherwise identical proteins were compared from different varieties. These differences persisted through different harvest years and in fruits grown in different Australian locations. With the definition of four different masses for PR-5 proteins (range = 21239−21272 Da) and nine different masses of PR-3 proteins (range = 25330−25631 Da) and using statistical analysis, the meth... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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