Effect of leech-centipede medicine on improving erectile function in diabetes-induced erectile dysfunction rats via PDE5 signalling pathway-related molecules

Autor: Zi-Long Chen, Haisong Li, Binghao Bao, Bin Wang, Sheng Deng, Xiao Li, Fanchao Meng, Junlong Feng, Hengheng Dai, Jisheng Wang
Rok vydání: 2021
Předmět:
Blood Glucose
Male
pathological change
animal structures
Pharmaceutical Science
Leech
Pharmacology
030226 pharmacology & pharmacy
01 natural sciences
Streptozocin
Diabetes Mellitus
Experimental
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
penis
Erectile Dysfunction
Diabetes mellitus
Drug Discovery
Medicine
Animals
Cyclic GMP
Cyclic Nucleotide Phosphodiesterases
Type 5
biology
business.industry
Tissue Extracts
Penile Erection
lcsh:RM1-950
biochemical indicator
General Medicine
Erectile function
biology.organism_classification
medicine.disease
Hedgehog signaling pathway
0104 chemical sciences
Rats
010404 medicinal & biomolecular chemistry
medicine.anatomical_structure
Erectile dysfunction
lcsh:Therapeutics. Pharmacology
Complementary and alternative medicine
Molecular Medicine
Nitric Oxide Synthase
business
Centipede
Diabetic Vascular Diseases
Penis
Signal Transduction
Research Article
Zdroj: Pharmaceutical Biology
article-version (VoR) Version of Record
Pharmaceutical Biology, Vol 59, Iss 1, Pp 167-174 (2021)
ISSN: 1744-5116
Popis: Context The leech and centipede granules have good curative effects on many diabetic vascular diseases, including diabetes-induced erectile dysfunction (DIED). Objective To explore the effect of leech and centipede on erectile function in rats with diabetes-induced erectile dysfunction and its possible mechanism. Materials and methods Thirty male Sprague–Dawley DIED rats were randomly divided into the model group (Group M), low-dose group (Group DD), high-dose group (Group DG) and tadalafil group (Group T) (n = 6); diabetic rats were induced by streptozotocin. Apomorphine was used to induce diabetic erectile dysfunction. The ‘leech-centipede’ granules (0.15 and 0.6 g/kg) were intragastrically administered in the DD and DG groups for 8 weeks. Blood glucose, serum insulin, testosterone, cGMP levels and protein expression changes were measured in each group. Results After 8 weeks, the erectile function of rats in the DG group significantly improved (1.26 ± 0.73). Penis tissue cGMP levels were higher in the DG group (1.48 ± 0.11) than in the M group (0.58 ± 0.15). Protein and mRNA expression levels of NOS were significantly higher (0.77 ± 0.05; 0.61 ± 0.02) but those of PDE5 (0.43 ± 0.05; 0.61 ± 0.03) were lower in the DG group than in the M group (0.37 ± 0.06; 0.51 ± 0.01; 0.78 ± 0.06; 0.81 ± 0.04). Conclusion The leech-centipede can improve erectile dysfunction in DIED rats by regulating the expression of cGMP, NOS, and PDE5-related molecules in the PDE5 pathway. This study provides a potential mechanism for the treatment of DIED with leech-centipede.
Databáze: OpenAIRE
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