Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos
| Autor: | Chandramallika Ghosh, Paul Collodi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
animal structures
biology Cell division Clinical Biochemistry Biomedical Engineering Bioengineering Embryo Cell Biology biology.organism_classification Blastula In vitro Culture Media Cell biology Trout Blastocyst Cell culture embryonic structures Immunology Animals Zebrafish Cell Division Cells Cultured Fetal bovine serum Biotechnology |
| Zdroj: | Cytotechnology. 14:21-26 |
| ISSN: | 1573-0778 0920-9069 |
| DOI: | 10.1007/bf00772192 |
| Popis: | The zebrafish has become a popular model for studies of vertebrate development and toxicology. However, in vitro approaches utilizing this organism have not been fully exploited due to the absence of suitable cell culture systems. Previously, we developed methods for the culture of cells derived from zebrafish blastula-stage embryos. One of these cultures, ZEM-2, was derived in a complex medium containing trout embryo extract, trout serum and medium conditioned by buffalo rat liver cells. In this study we describe a zebrafish embryo cell line, ZEM-2A, derived from ZEM-2 following selection for growth in a simplified medium. Optimal growth of ZEM-2A cells is attained in nutrient medium supplemented with 5% fetal bovine serum. |
| Databáze: | OpenAIRE |
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