| Autor: |
Yashan Deng, Xixi Wang, Hui Xu, Cui Liu, Ran Li, Yuanyuan Zhang, Changfeng Qu, Jinlai Miao |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Molecules; Volume 27; Issue 22; Pages: 7716 |
| DOI: |
10.21203/rs.3.rs-2070747/v1 |
| Popis: |
The bioenzymatic production of selenium oligosaccharides addresses the problems resulting from high molecular weight and poor water solubility of κ-selenocarrageenan, and lays foundation for its application as adjuvant drugs for cancer treatment and food additive. κ-selenocarrageenase extracted from Pseudoalteromonas sp. Xi13 can degrade κ-selenocarrageenan to selenium oligosaccharides. The maximum optimized κ-selenocarrageenase activity using Response Surface Methodology (RSM) was increased by 1.4 times, reaching 8.416 U/mL. To expand applications of the κ-selenocarrageenase in industry, the preparation conditions of it in either lyophilized or immobilized form were investigated. The activity recovery rate of the lyophilized enzyme was >70%, while that of the immobilized enzyme was 62.83%. However, the immobilized κ-selenocarrageenase exhibits good stability after being reused four times, with 58.28% of residual activity. The selenium content of κ-selenocarrageenan oligosaccharides degraded by the immobilized κ-selenocarrageenase was 47.06 µg/g, 8.3% higher than that degraded by the lyophilized enzyme. The results indicate that the immobilized κ-selenocarrageenase is suitable for industrial applications and has commercial potential. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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