Genetic characterization of a novel recombinant PRRSV2 from lineage 8, 1 and 3 in China with significant variation in replication efficiency and cytopathic effects

Autor: Fang He, Kai-Xia Lei, Guangwei Han, Huiling Xu
Rok vydání: 2020
Předmět:
China
Lineage (genetic)
Swine
040301 veterinary sciences
Porcine Reproductive and Respiratory Syndrome
Genome
Viral
Virus Replication
medicine.disease_cause
Genome
Genetic recombination
law.invention
0403 veterinary science
03 medical and health sciences
Cytopathogenic Effect
Viral
law
Macrophages
Alveolar
medicine
Animals
Porcine respiratory and reproductive syndrome virus
Amino Acid Sequence
Phylogeny
030304 developmental biology
Recombination
Genetic
Swine Diseases
Genetics
0303 health sciences
Mutation
Base Sequence
General Veterinary
General Immunology and Microbiology
biology
Phylogenetic tree
Nucleic acid sequence
Genetic Variation
Genomics
04 agricultural and veterinary sciences
General Medicine
Porcine reproductive and respiratory syndrome virus
biology.organism_classification
Recombinant DNA
Zdroj: Transboundary and Emerging Diseases. 67:1574-1584
ISSN: 1865-1682
1865-1674
DOI: 10.1111/tbed.13491
Popis: There are four major porcine reproductive and respiratory syndrome virus 2 (PRRSV2) lineages circulating in China based on classification system, including lineages 1 (NADC30-like), 3 (QYYZ-like), 5.1 (VR2332-like) and 8 (JXA1-like/CH-1a-Like), which leads to the potential recombination. In the present study, a novel variant of PRRSV2 strain named JS18-3 was isolated from piglets suffering severe breathing difficulties in Jiangsu Province of China in 2018. Full-length genome analysis indicated that JS18-3 shared 86.5%, 87.9%, 84.2%, 82.2% and 86.4% nucleotide similarity with PRRSVs CH-1a, JXA1, VR2332, QYYZ and NADC30, respectively. 4871-6635 of JS18-3 shared the highest identity of 99.3% in nucleotide sequence with HP-PRRSV representative strain JXA1 indicating ongoing evolution to HP-PRRSV. JS18-3 was classified into classical lineage 8 of PRRSV2 based on phylogenetic analysis of complete genome and ORF5. Genomic break points in structural (ORF3) and non-structural (NSP2, NSP3) regions of genomes were detected in recombination analysis. JS18-3 is a recombinant isolate from lineages 8, 1 and 3. Replication enhancement and severe cytopathic effects caused by JS18-3 were observed in Marc-145 cells and porcine alveolar macrophages (PAMs) as compared to JX07, a typical strain of lineage 8. Pathogenicity results indicated that piglets inoculated with JS18-3 presented persistent fever, dyspnoea, serious microscopic lung lesions and lymph node congestion. The study suggests that lineage 8 of PRRSV2 is involved in continuing evolution by genetic recombination and mutation leading to outbreaks in vaccinated pigs in China.
Databáze: OpenAIRE
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