Interferon-γ–inducible protein p16. a new target of antinuclear antibodies in patients with systemic lupus erythematosus
| Autor: | H. P. Seelig, Manfred Renz, Hans Ehrfeld |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Anti-nuclear antibody
Immunoblotting Molecular Sequence Data Immunology Fluorescent Antibody Technique Immunofluorescence Autoantigens Epitopes Interferon-gamma Rheumatology Antigen Gene expression medicine Animals Humans Lupus Erythematosus Systemic Immunology and Allergy Pharmacology (medical) Interferon gamma RNA Messenger Lupus erythematosus Base Sequence medicine.diagnostic_test biology Autoantibody Nuclear Proteins Proteins Blotting Northern Phosphoproteins medicine.disease Precipitin Tests Antibodies Antinuclear biology.protein Rabbits Antibody medicine.drug |
| Zdroj: | Arthritis & Rheumatism. 37:1672-1683 |
| ISSN: | 1529-0131 0004-3591 |
| Popis: | Objective To determine the cellular expression and localization of interferon-gamma (IFN gamma)-inducible protein p16, a new antigen specificity of antinuclear antibodies (ANA), and to evaluate the prevalence of anti-p16, particularly in SLE patients. Methods Serum levels of anti-p16 were determined by immunoblotting with recombinant p16 and cellular p16 messenger RNA (mRNA) by Northern blotting. We also utilized immunoprecipitation of 35S-methionine-labeled proteins, immunostaining of blotted proteins of subcellular fractions, and immunofluorescence studies with affinity-purified rabbit and human anti-recombinant p16. Results Protein p16 was localized within the nucleolus and nucleoplasm and constitutively expressed in Raji, HeLa, HEp-2, K562, and HL-60 cells. Synthesis of mRNA and protein was increased in the presence of IFN gamma. The prevalence of anti-p16 was 29% in 374 SLE patients (35% in those positive for anti-double-stranded DNA) and 0% in 188 healthy individuals. Anti-p16 was always accompanied by positive findings on indirect immunofluorescence for ANA. Conclusion Anti-p16 represents a main ANA specificity. Anti-p16 may help to elucidate IFN gamma-dependent nuclear processes and to link autoantibody production to states of IFN gamma activation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text