Effect of an MG132-Sustained Drug Delivery Capsular Ring on the Inhibition of Posterior Capsule Opacification in a Rabbit Model
| Autor: | Min Hou, Mingxing Wu, Yingyan Qin, Xuan Bao, Furong Luo, F Shang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Leupeptins medicine.medical_treatment macromolecular substances Immunofluorescence 03 medical and health sciences chemistry.chemical_compound Entrapment 0302 clinical medicine Drug Delivery Systems Western blot Polylactic Acid-Polyglycolic Acid Copolymer Ophthalmology MG132 medicine Animals Pharmacology (medical) Lactic Acid Pharmacology medicine.diagnostic_test Phacoemulsification Anatomy Capsule Opacification In vitro PLGA Disease Models Animal 030104 developmental biology chemistry Drug delivery 030221 ophthalmology & optometry Posterior Capsule of the Lens Rabbits Polyglycolic Acid |
| Zdroj: | Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics. 33(2) |
| ISSN: | 1557-7732 |
| Popis: | To design an MG132-sustained drug delivery capsular ring (SDDCR) and investigate its effect on the inhibition of posterior capsule opacification (PCO) in a rabbit model.The SDDCRs were prepared by forming a slice of film made by the mixture of poly lactic-co-glycolic acid (PLGA) and MG132 on the surface of capsular tension rings (CTRs). The drug-loading capacity, entrapment efficiency, and in vitro release of the drug-containing film were detected. Eighteen New Zealand white rabbits were operated with phacoemulsification and MG132-SDDCRs/PLGA-CTRs/CTRs implantation in the single eye. The images of the anterior segments were acquired at certain days, and the epithelial-mesenchymal transition (EMT) markers were detected by western blot and immunofluorescence.The drug-loading capacity and entrapment efficiency of MG132-SDDCRs were 1.15% ± 0.04% and 66.16% ± 0.027%, respectively, and the drug released well within a month. The PCO degree of the MG132-SDDCR group was significantly lower than the other groups. The expression of alpha-smooth muscle actin, fibronectin, vimentin, and collagen-I was lower, and the expression of E-cadherin (E-cad) was higher in the MG132-SDDCR group than the other groups.MG132-SDDCRs could be established successfully. The PCO process was prevented, and the expression of EMT markers was inhibited by the implantation of MG132-SDDCRs, indicating that this could be a potential treatment against PCO. |
| Databáze: | OpenAIRE |
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