Predominant Expression of Murine Bmx Tyrosine Kinase in the Granulo-Monocytic Lineage

Autor: Chung Leung Li, Dominique Weil, Maryanne Power, Shirley I. Smith
Přispěvatelé: Compartimentation et trafic intracellulaire des mRNP = Compartmentation and intracellular traffic of mRNPs (LBD-E14), Laboratoire de Biologie du Développement [Paris] (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 1997
Předmět:
Adult
Neutrophils
Cellular differentiation
Molecular Sequence Data
Population
Immunology
Cell Separation
Biology
Biochemistry
Monocytes
Mice
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Bruton's tyrosine kinase
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Amino Acid Sequence
Cloning
Molecular
Progenitor cell
education
030304 developmental biology
education.field_of_study
0303 health sciences
Macrophages
Hematopoietic stem cell
Cell Differentiation
Cell Biology
Hematology
Protein-Tyrosine Kinases
Flow Cytometry
Hematopoietic Stem Cells
Molecular biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cancer research
biology.protein
Stem cell
Tyrosine kinase
Granulocytes
030215 immunology
Proto-oncogene tyrosine-protein kinase Src
Zdroj: Europe PubMed Central
Blood
Blood, American Society of Hematology, 1997, 90 (11), pp.4332-40
ISSN: 1528-0020
0006-4971
Popis: In the course of systematic cloning of protein tyrosine kinases (PTKs) expressed in hematopoietic stem and progenitor cells, we have identified the murine homologue of human Bmx. It encodes a protein containing the five domains characteristic of the Tec family of cytoplasmic src-related PTKs: pleckstrin homology (PH), Tec homology (TH), src homology 3 and 2 (SH3 and SH2), and tyrosine kinase (TK). In adults, Bmx expression was found primarily in bone marrow and at a lower level in lung and heart. During fetal development it was also found in the spleen at late stage of gestation and in neonates. Analysis of bone marrow subpopulations showed that Bmx was expressed in the progenitor cell population and maturing hematopoietic cells of the granulo/monocytic lineage where expression increased with maturation and differentiation. At the periphery, a high level of Bmx expression was also found in neutrophils and monocytes/macrophages. Bmx expression was not detected in the primitive hematopoietic stem cell population, and cells of the B-, T-, and erythroid-lineages. It was also not detected in most of the cell lines examined. Our results indicate that Bmx is another member of the Btk/Itk/Tec PTK family, which is predominantly expressed in the granulo-monocytic lineage within the hematopoietic system.
Databáze: OpenAIRE
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