Beneficial effects of metformin on muscle atrophy induced by obesity in rats
| Autor: | Mai M. Hasan, Eman M. A. Abdelghany, Jehan El‐Gendy, Sally M. Shalaby |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty medicine.medical_treatment Diet High-Fat Biochemistry 03 medical and health sciences 0302 clinical medicine Internal medicine Myosin medicine Animals Hypoglycemic Agents Obesity Molecular Biology Soleus muscle business.industry Insulin Forkhead Box Protein O3 Cell Biology medicine.disease Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Muscle atrophy Metformin Rats Muscular Atrophy 030104 developmental biology Endocrinology Gene Expression Regulation 030220 oncology & carcinogenesis FOXO3 Homeostatic model assessment medicine.symptom business medicine.drug Signal Transduction |
| Zdroj: | Journal of cellular biochemistry. 120(4) |
| ISSN: | 1097-4644 |
| Popis: | AIM A growing interest to understand the signaling pathways mediating obesity-induced muscle atrophy is given. Metformin (Met) was reported to possess positive effects on preventing muscle damage and promoting muscle mass maintenance. The aim of the present study to investigate pathways involved in Met effect on obesity induced muscle atrophy. METHODS Thirty adult male albino rats were assigned into two groups: normal chew diet fed group as control group (C; n = 10) and high-fat-diet (HFD) fed group ( n = 20). After 16 weeks, the HFD-fed animals were subdivided into two groups; HFD group ( n = 10) and HFD fed treated with oral Met (320 mg/day) treatment (Met, n = 10) for 4 weeks. At the end of the experiment; final body weight, visceral fat weight, fasting blood glucose, insulin, lactate, total cholesterol, triglycerides were measured and calculated homeostatic model assessment insulin resistant (HOMA-IR) for all groups. Soleus muscle weight, histopathlogical examination and expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), forkhead box O3 (FoxO3), atrogin-1/MAFbx, and muscle RING finger 1 (MuRF-1) were performed. RESULTS HFD-fed animals showed significant increase in final body weight, visceral fat mass, fasting blood glucose, insulin, calculated HOMA-IR, lactate, total cholesterol and triglycerides with significant decrease in soleus muscle weight, PGC-1α and significant increase in FoxO3, atrogin-1/MAFbx, and MuRF-1 expression. Also, there was significant decrease in fiber diameter, myosin heavy chain (MHC) I content while collagen content and myosin heavy chain IIa were increased compared with control group. Met-treated group showed a significant decrease in the measured parameters compared with the HFD group. It also restored the gene expression, morphometric measures and MHC composition toward normal. CONCLUSION The current study is the first to provide evidence that Met could ameliorate muscle atrophy in high-fat diet induced obesity and this effect may be in part due to regulation of PGC-1α-FoxO3 pathway. |
| Databáze: | OpenAIRE |
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