Enzyme immunoassay for the identification of hemoglobin variants
| Autor: | C. R. Kiefer, F. A. Garver, M Shyamala, Hugo Moscoso |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
Hemoglobins Abnormal Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Immunoenzyme Techniques Antibody Specificity medicine Animals Humans Genetics (clinical) Antiserum chemistry.chemical_classification Hemoglobin J biology medicine.diagnostic_test Isoelectric focusing Immune Sera Biochemistry (medical) Infant Newborn Hemoglobin variants Hematology medicine.disease Molecular biology Enzyme Hemoglobinopathy chemistry Immunoassay biology.protein Rabbits Antibody Isoelectric Focusing Peroxidase |
| Zdroj: | Hemoglobin. 14(4) |
| ISSN: | 0363-0269 |
| Popis: | We have prepared monospecific antibodies to Hbs D-Los Angeles, J-Baltimore, O-Arab and J-Paris-I and developed an enzyme immunoassay (ELISA) for their identification in hemolysates. Hbs in adult or cord blood hemolysates were coated to the wells of microtiter plates and reacted with the appropriate antisera followed by the detection system which contains anti-rabbit IgG/peroxidase conjugate and the substrate tetramethylbenzidine. Sixty-nine samples were tentatively considered to contain the above hemoglobin variants by isoelectrofocusing and the identity of 83% of them was confirmed by ELISA. Some of the non-reacting hemolysates were shown by amino acid sequence analysis to contain Hbs Korle-Bu, D-Ibadan, G-Copenhagen and the new variant Chandigarh. This ELISA offers specificity and simplicity for the confirmatory identification of hemoglobin variants. |
| Databáze: | OpenAIRE |
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