Yeast two-hybrid screens imply involvement of fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport
| Autor: | Annette L. Medhurst, Christopher G. Mathew, Maureen E. Hoatlin, Pia A. J. Huber, Yu Zhi, Sabine Herterich, Tanja Reuter, Hans J. Gross, Hans Joenje, Holger Hoehn, Quinten Waisfisz |
|---|---|
| Přispěvatelé: | Human genetics, CCA - Cancer biology and immunology, CCA - Imaging and biomarkers |
| Rok vydání: | 2003 |
| Předmět: |
DNA
Complementary Fanconi anemia complementation group C Two-hybrid screening DNA Mutational Analysis Cell Cycle Proteins Saccharomyces cerevisiae Biology DNA-binding protein Oxidative Phosphorylation Fanconi anemia FANCG Two-Hybrid System Techniques hemic and lymphatic diseases Genes Regulator Escherichia coli medicine Humans Fanconi Anemia Complementation Group G Protein Cell Cycle Protein Cells Cultured Genetics Fanconi Anemia Complementation Group A Protein Fanconi Anemia Complementation Group C Protein Nuclear Proteins Proteins Cell Biology medicine.disease Fanconi Anemia Complementation Group Proteins FANCA Transport protein DNA-Binding Proteins Protein Transport Fanconi Anemia Signal Transduction |
| Zdroj: | Experimental Cell Research, 289(2), 211-221. Academic Press Inc. Reuter, TY, Medhurst, AL, Waisfisz, Q, Zhi, Y, Herterich, S, Hoehn, H, Gross, HJ, Joenje, H, Hoatlin, ME, Mathew, CG & Huber, PA 2003, ' Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport ', Experimental Cell Research, vol. 289, no. 2, pp. 211-221 . https://doi.org/10.1016/S0014-4827(03)00261-1 |
| ISSN: | 0014-4827 |
| Popis: | Mutations in one of at least eight different genes cause bone marrow failure, chromosome instability, and predisposition to cancer associated with the rare genetic syndrome Fanconi anemia (FA). The cloning of seven genes has provided the tools to study the molecular pathway disrupted in Fanconi anemia patients. The structure of the genes and their gene products provided few clues to their functional role. We report here the use of 3 FA proteins, FANCA, FANCC, and FANCG, as "baits" in the hunt for interactors to obtain clues for FA protein functions. Using five different human cDNA libraries we screened 36.5x10(6) clones with the technique of the yeast two-hybrid system. We identified 69 proteins which have not previously been linked to the FA pathway as direct interactors of FANCA, FANCC, or FANCG. Most of these proteins are associated with four functional classes including transcription regulation (21 proteins), signaling (13 proteins), oxidative metabolism (10 proteins), and intracellular transport (11 proteins). Interaction with 6 proteins, DAXX, Ran, IkappaBgamma, USP14, and the previously reported SNX5 and FAZF, was additionally confirmed by coimmunoprecipitation and/or colocalization studies. Taken together, our data strongly support the hypothesis that FA proteins are functionally involved in several complex cellular pathways including transcription regulation, cell signaling, oxidative metabolism, and cellular transport. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect