Overexpression of CBS and CSE genes affects lifespan, stress resistance and locomotor activity in Drosophila melanogaster
| Autor: | M. Shaposhnikov, Lyubov А. Koval, Alex Zhavoronkov, Ekaterina Proshkina, Alexey Moskalev, Nadezhda Zemskaya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
inorganic chemicals Male Aging congenital hereditary and neonatal diseases and abnormalities Transgene paraquat Longevity hydrogen sulfide Cystathionine beta-Synthase Motor Activity medicine.disease_cause Gene Expression Regulation Enzymologic 03 medical and health sciences chemistry.chemical_compound desiccation 0302 clinical medicine Paraquat Stress Physiological parasitic diseases medicine Animals Gene chemistry.chemical_classification biology organic chemicals starvation Cystathionine gamma-Lyase nutritional and metabolic diseases Cell Biology biology.organism_classification hyperthermia Cystathionine beta synthase Cell biology 030104 developmental biology Enzyme Drosophila melanogaster chemistry Gene Expression Regulation Cytoplasm 030220 oncology & carcinogenesis biology.protein Drosophila Female locomotor activity Oxidative stress lifespan Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | Recent experimental studies highlighted the role of hydrogen sulfide (H2S) in aging and longevity. The cystathionine s-synthase (CBS) and cystathionine γ-lyase (CSE) are the key enzymes responsible for H2S production. Here we investigated the geroprotective effects of CSE and CBS overexpression in Drosophila. Overexpression of CSE did not affect a lifespan and decrease (mitochondrial form of CSE) or increase (cytoplasmic form of CSE) age dynamics of locomotor activity, while overexpression of CBS increase median (by 12.5%) and maximum (by 6.9%) lifespan and locomotor activity. Increasing of both CSE and CBS expression levels resulted in thermotolerance, but the resistance to combination of arid and food-free conditions decreased. The resistance to oxidative stress (paraquat) was not affected in flies with overexpression of CBS and cytoplasmic CSE, but decreased in flies overexpressing mitochondrial form of CSE. Thus, transgene overexpression of the CSE and CBS in Drosophila induce similar effects on stress-resistance and locomotor activity, however lifespan extending effect was revealed for CBS overexpression only. |
| Databáze: | OpenAIRE |
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