Measuring Traction Forces of Motile Dendritic Cells on Micropost Arrays
| Autor: | Christopher S. Chen, Brendon G. Ricart, Daniel A. Hammer, Christopher A. Hunter, Michael T. Yang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Leading edge
Time Factors Surface Properties medicine.medical_treatment Biophysics Chemokinesis Motility 02 engineering and technology Biology 03 medical and health sciences Mice Cell Movement medicine Animals Cellular Biophysics and Electrophysiology Pseudopodia 030304 developmental biology 0303 health sciences Mesenchymal stem cell Chemotaxis Anatomy Actomyosin Dendritic Cells Traction (orthopedics) Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Biomechanical Phenomena Chemokine CCL19 Stress Mechanical 0210 nano-technology Filopodia |
| Zdroj: | Biophysical Journal. 101(11):2620-2628 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2011.09.022 |
| Popis: | Dendritic cells (DCs) migrate from sites of inflammation to secondary lymphoid organs where they initiate the adaptive immune response. Although motility is essential to DC function, the mechanisms by which they migrate are not fully understood. We incorporated micropost array detectors into a microfluidic gradient generator to develop what we consider to be a novel method for probing low magnitude traction forces during directional migration. We found migration of primary murine DCs is driven by short-lived traction stresses at the leading edge or filopodia. The traction forces generated by DCs are smaller in magnitude than found in neutrophils, and of similar magnitude during chemotaxis and chemokinesis, at 18 ± 1.4 and 16 ± 1.3 nN/cell, respectively. The characteristic duration of local DC traction forces was 3 min. The maximum principal stress in the cell occurred in the plane perpendicular to the axis of motion, forward of the centroid. We illustrate that the spatiotemporal pattern of traction stresses can be used to predict the direction of future DC motion. Overall, DCs show a mode of migration distinct from both mesenchymal cells and neutrophils, characterized by rapid turnover of traction forces in leading filopodia. |
| Databáze: | OpenAIRE |
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