Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells
| Autor: | Elżbieta Pamuła, Małgorzata Krok-Borkowicz, Vera Hintze, Łucja Rumian, Ricardo Bernhardt, Iwona M. Wojak-Ćwik, Ricarda Hess, Matthias Schnabelrauch, Piotr Dobrzyński, Dieter Scharnweber, Stephanie Möller |
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| Rok vydání: | 2018 |
| Předmět: |
Bone sialoprotein
Adult Calcium Phosphates Male Materials science Bioengineering Core Binding Factor Alpha 1 Subunit 02 engineering and technology 010402 general chemistry 01 natural sciences Collagen Type I Biomaterials chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Osteogenesis Extracellular Cell Adhesion Humans Integrin-Binding Sialoprotein Osteopontin Hyaluronic Acid Bone regeneration Sirius Red Cell Proliferation biology Tissue Engineering Tissue Scaffolds Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells 021001 nanoscience & nanotechnology 0104 chemical sciences Extracellular Matrix PLGA chemistry Mechanics of Materials biology.protein Biophysics Microscopy Electron Scanning Surface modification 0210 nano-technology |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 97 |
| ISSN: | 1873-0191 |
| Popis: | The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering. Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC). Moreover, a new cell seeding method assuring 90% seeding efficiency on the scaffolds was developed. Poly( l ‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya). The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography. Two types of pores with diameters 400–600 μm and 2–20 μm were obtained. The scaffolds were successfully coated with a homogenous layer of aECM as shown by Sirius red and toluidine blue staining. In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution. However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II. As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration. |
| Databáze: | OpenAIRE |
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