Composition of the Stabilizer and Conditions of Lyophilization for Preserving Infectious Activity of Influenza Virus
| Autor: | Irina Kiseleva, I. A. Dubrovina, E. V. Kireeva, Larisa Rudenko |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sucrose 02 engineering and technology General Biochemistry Genetics and Molecular Biology Virus Madin Darby Canine Kidney Cells 03 medical and health sciences chemistry.chemical_compound Dogs Drug Stability Animal proteins Animals Food science General Medicine Orthomyxoviridae 021001 nanoscience & nanotechnology Trehalose Freeze Drying 030104 developmental biology chemistry Influenza Vaccines Peptones Composition (visual arts) 0210 nano-technology Stabilizer (chemistry) |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 165:52-56 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-018-4097-7 |
| Popis: | For stabilization of vaccine preparations, they are lyophilized. The composition of the protective medium is an important parameter affecting the quality of the vaccine after drying. In view of the risk of spreading prion diseases, the use of media containing animal proteins is not recommended. In this study, protective media containing no animal proteins and lyophilization regimen were determined. The optimum lyophilization regimen consisted of three stages: freezing at -70°C, main stage at -35°C, and drying at 24°C. Protective medium containing 4% trehalose or protective medium with 10% sucrose and 5% soy peptone ensured highest stability of the lyophilized vaccine preparation in temperature range of 4-24°C. This can help to overcome possible break in the cold chain, which is important during transporting or storage of vaccine preparations. |
| Databáze: | OpenAIRE |
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