Evaluation of the direct agglutination test and the rK39 dipstick test for the sero-diagnosis of visceral leishmaniasis
| Autor: | Eduardo Silva, Marilene M. Canto-Cavalheiro, Henk D. F. H. Schallig |
|---|---|
| Přispěvatelé: | KIT: Biomedical Research, Medical Microbiology and Infection Prevention |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine diagnosis lcsh:RC955-962 Protozoan Proteins Leishmania donovani lcsh:QR1-502 Antibodies Protozoan Cold storage Antigens Protozoan direct agglutination test Sensitivity and Specificity lcsh:Microbiology Agglutination Tests Direct agglutination test mental disorders parasitic diseases medicine Animals Humans visceral leishmaniasis human biology business.industry Leishmania chagasi Reproducibility of Results Leishmaniasis Dipstick biology.organism_classification medicine.disease Freeze Drying Visceral leishmaniasis rK39 dipstick test Case-Control Studies Immunology biology.protein Leishmaniasis Visceral Antibody business Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 97, Iss 7, Pp 1015-1018 (2002) Memórias do Instituto Oswaldo Cruz, Volume: 97, Issue: 7, Pages: 1015-1018, Published: OCT 2002 Memorias do Instituto Oswaldo Cruz, 97(7), 1015-1018. Fundacao Oswaldo Cruz |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | The direct agglutination test (DAT) based on a freeze-dried antigen and the rK39 dipstick test were evaluated for the sero-diagnosis of visceral leishmaniasis (VL). The sensitivity and specificity of both tests were determined using sera from confirmed VL patients (n = 21), healthy controls (n = 19) and from patients with other confirmed infectious diseases (n = 42). The DAT had a sensitivity and a specificity of 100%. The rK39 had a sensitivity of 85.7% and a specificity of 82%. Both tests were also used to screen blood samples of confirmed VL patients (n = 15) and serum samples of VL suspects (n = 61). The DAT found all blood samples of confirmed VL patients positive and tested 98.4% of the serum samples of the VL suspects positive. In contrast, rK39 detected in 9/15 blood samples (60%) antibodies against Leishmania chagasi and found 85.3% of the serum samples of the suspected patients positive. Although the rK39 dipstick is more rapid and user friendlier than the DAT, the latter has a superior sensitivity and specificity. Furthermore, the reagents used for DAT do not require cold storage, whereas the buffer of the rK39 must be stored at 4oC. Therefore, the DAT is the most suitable test for the sero-diagnosis of VL under field conditions. |
| Databáze: | OpenAIRE |
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