Investigation of vinca alkaloid-plasma membrane interactions by detergent gel chromatography
| Autor: | L. Holczinger, T. Kremmer |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Male
Sodium Protein subunit chemistry.chemical_element Biochemistry Micelle Analytical Chemistry Gel permeation chromatography chemistry.chemical_compound Animals Vinca Alkaloids Chromatography Elution Liver cell Cell Membrane Organic Chemistry Proteins General Medicine Lipids Rats Membrane Liver chemistry Vincristine Chromatography Gel Agarose Protein Binding |
| Zdroj: | Journal of Chromatography A. 191:287-292 |
| ISSN: | 0021-9673 |
| Popis: | Detergent gel chromatography was successfully applied for the separation of protein subunits and lipid micelles of [14C]Vincristine-treated and sodium dodecyl sulphate-solubilized liver cell plasma membranes. The elution profiles of solubilized membranes depended on the pore size of the commercial agarose and agarose-polyacrylamide gels used. It was found that most of the membrane-bound Vincristine was associated with the solubilized lipid micelles and only a very small proportion was bound to protein subunits. |
| Databáze: | OpenAIRE |
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