Analysis of the Circular Transcriptome in the Synaptosomes of Aged Mice
Autor: | Konii Takenaka, Ludmil Kirazov, Ivonne Zipfel, Martina K. Brückner, Jennifer Jünger, Jana Bochmann, Uwe Ueberham, Bei Jun Chen, Ashton Curry-Hyde, James D. Mills, E. Kirazov, Renate Jendrek, Thomas Arendt, Michael Janitz |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Gene regulatory network Neurotransmission Biology Transcriptome 03 medical and health sciences 0302 clinical medicine Aging brain Animals Gene Regulatory Networks Gene synaptosome General Neuroscience Brain RNA Circular Cell biology 030104 developmental biology Ageing mouse brain RNA splicing Proteome RNA RNA-seq aging brain 030217 neurology & neurosurgery circular RNAs Synaptosomes |
Zdroj: | Neuroscience. 449 |
ISSN: | 1873-7544 |
Popis: | Recently, circular RNAs (circRNAs) have been revealed to be an important non-coding element of the transcriptome. The brain contains the most abundant and widespread expression of circRNA. There are also indications that the circular transcriptome undergoes dynamic changes as a result of brain ageing. Diminished cognitive function with increased age reflects the dysregulation of synaptic function and ineffective neurotransmission through alterations of the synaptic proteome. Here, we present changes in the circular transcriptome in ageing synapses using a mouse model. Specifically, we observed an accumulation of uniquely expressed circular transcripts in the synaptosomes of aged mice compared to young mice. Individual circRNA expression patterns were characterized by an increased abundance in the synaptosomes of young or aged mice, whereas the opposite expression was observed for the parental gene linear transcripts. These changes in expression were validated by RT-qPCR. We provide the first comprehensive survey of the circular transcriptome in mammalian synapses, thereby paving the way for future studies. Additionally, we present 16 genes that express solely circRNAs, without linear RNAs co-expression, exclusively in young and aged synaptosomes, suggesting a synaptic gene network that functions along canonical splicing activity. |
Databáze: | OpenAIRE |
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