Electron flow from PSII to PSI under high light is controlled by PGR5 but not by PSBS
| Autor: | Mikko Tikkanen, Sanna Rantala, Eva-Mari Aro |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
thermal dissipation control of Cyt b6f NPQ cyclic electron transfer Plastoquinone macromolecular substances Plant Science lcsh:Plant culture Photosystem I 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Electron transfer Botany lcsh:SB1-1110 Electrochemical gradient 030304 developmental biology Original Research 0303 health sciences P700 Cytochrome b6f complex Chemistry food and beverages trans-thylakoid proton gradient Chloroplast trans thylakoid proton gradient Light intensity Biophysics regulation of photosynthetic electron transfer chain P700 oxidation 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 6 (2015) |
| ISSN: | 1664-462X |
| Popis: | Absence of the Proton Gradient Regulation 5 (PGR5) protein from plant chloroplasts prevents the induction of strong trans-thylakoid proton gradient (ΔpH) and consequently also the thermal dissipation of excess energy (NPQ). The absence of the PSBS protein likewise prevents the formation of ΔpH-dependent NPQ. This component of NPQ is called qE, which is nearly exclusively responsible for induction of NPQ upon increase in light intensity. On the other hand, the pgr5 mutant is not only deficient in induction of strong NPQ but it also lacks the capability to oxidize P700 upon increase in light intensity. This, in turn, results from uncontrolled electron flow toward photosystem I (PSI), which has been proposed to be caused by the lack of PSII down-regulation by NPQ and by a poor control of electron flow via the Cytochrome b6f (Cyt b6f) complex. Here we asked whether NPQ really is a component of such regulation of electron flow from PSII to PSI at high light. To this end, the two NPQ mutants pgr5 and npq4, the latter lacking the PSBS protein, were characterized. It is shown that the npq4 mutant, despite its highly reduced Plastoquinone pool, does not inhibit but rather enhances the oxidation of P700 in high light as compared to wild type. This clearly demonstrates that the control of electron flow from PSII to PSI cannot be assigned, even partially, to the down-regulation of PSII by NPQ but apparently takes place solely in Cyt b6f. Moreover, it is shown that the pgr5 mutant can induce NPQ in very high light, but still remains deficient in P700 oxidation. These results challenge the suggestion that NPQ, induced by PGR5-dependent cyclic electron transfer, would have a key role in regulation of electron transfer from PSII to PSI. Instead, the results presented here are in line with our recent suggestion that both PSII and PSI function under the same light harvesting machinery regulated by ΔpH and the PSBS protein (Tikkanen and Aro, 2014; Grieco et al., 2015). |
| Databáze: | OpenAIRE |
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